INTRODUCE OF EFFECTIVE ANTIBIOTICS AND VACCINES ON FORAGE CROPS BY MEANS OF GENETIC ENGINEERING

我们改进了我们以前发表的从百喜草种子胚性愈伤组织再生植株的技术，通过规则的分区保持愈伤组织的小尺寸。这种改良的培养物保持可再生至少18个月，并导致再生植株中白化病植株的减少。此外，在转化实验中，增加的表面积可用于基因递送，并且更多数量的细胞可以潜在地再生。使用一个简单的自建粒子流入枪与改进的技术特点，我们已经优化了传递条件和轰击的小愈伤组织与pDB 1，构建携带GUS报告基因和双丙氨磷抗性基因（酒吧）。在补充双丙氨膦的培养基上选择了大量愈伤组织，并使其经受再生条件。这些愈伤组织保持抗性并继续显示GUS活性。抗性愈伤组织在再生条件下形成的芽和胚状结构以及绿色植株 关于我们 百脉根超级根是一种快速生长的豆科植物根培养物，其允许在完全无生长调节剂的培养条件下连续的根克隆、直接体细胞胚胎发生和植株的大量再生。这些特征是根培养物所特有的，并且自从近5年前分离培养物以来，现在稳定表达。在几年的实验中，我们所有用农杆菌直接转化超级根的尝试都失败了，原因不明。直到最近，通过使用超级根来源的愈伤组织作为受体组织，我们才能够证明报告基因（GUS）在超级根来源的组织中的瞬时表达。在转化愈伤组织再生的根和芽中也可见GUS表达。超级根性状在整个愈伤组织阶段保持稳定。从这些愈伤组织衍生的根可以作为新的超级根培养的外植体。愈伤组织阶段的这种稳定性使得转化不太可能是通过失去保护性遗传信息而实现的，例如自发的A。通过愈伤组织的诱导，使发根菌感染。少

We have improved our previously published technique for plant regenerationfrom seed-derived embryogenic callus of bahiagrass by keeping the callus size small through regular partition. This modifiedculture remains regenerable for at least 18 months and results in a decrease of albino plants among regenerants. Also, in transformation experiments, an increased surface area is available for gene delivery, and a greater number of cells can potentially regenerate. Using a simple self-built particle inflow gun with improved technical features, we have optimized delivery conditions and bombarded the small calli with pDB1, a construct carrying both the GUS reporter gene and a bialaphos resistance gene (bar). Numerous calli have been selected on bialaphos-supplemented medium and subjected to regeneration conditions. These calli remained resistant and continue to display GUS activity. Shoots as well as embryo-like structures formed from resistant calli under regeneration conditions and green pla … More nts were obtained.Super roots of Lotus corniculatus are a fast growing legume root culture that allows continuous root cloning, direct somatic embryogenesis and mass regeneration of plants under entirely growth regulator-free culture conditions. These features are unique to root cultures and are now stable expressed since the, culture has been isolated almost 5 years ago. In several years of experimentation, all our attempts to directly transform super roots with Agrobacterium failed for unknown reasons. Only recently, by using super root-derived callus as acceptor tissue, we were able to demonstrate transient expression of a reporter gene (GUS) in a tissuederived from super roots. GUS expression was also, visible in roots and shoots regenerated from transformed calli. The super root character is stable maintained through the callus phase. Roots derived from these calli can serve as explants for new super root cultures. This stability of the callus phase makes it unlikely that transformation was made possible through the loss of protective genetic information, such as a spontaneous A. rhizogenes infection, through callus initiation. Less