Analysis on allergenic 13S globulin subunits of buckwheat

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Buckwheat genomic DNA library, consisting of 142,005 clones, was screened to elucidate the diversity of 13S globulin genes. Twenty-six BAC clones were isolated and 17 genes of 13S globulin were identified, the sequences of which were deposited into DNA Data Bank of Japan. The unit length of nucleotides for most tandem repeat sequences, which are specifically inserted into alpha polypeptides of 13S globulin, was 45 bp while that of some repeats had extra three nucleotides like CGG or AGA, resulting in different secondary structure formation. On the other hand, preliminary experimental system was developed to analyze the effect of environmental factors on 13S globulin composition using autogamous tartary buckwheat.