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Analysis of dsRNA mechanism of response in insect cell

Analysis of dsRNA mechanism of response in insect cell
昆虫细胞中双链RNA反应机制分析
批准号:
23580077
负责人:
LEE JAEMAN
金额:
$3.41万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2011
资助国家:
日本
项目状态:
已结题
起止时间:
2011 至 2013

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中文摘要
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英文摘要
In order to establish efficient RNA interference induction system in insect cells, we introduced several membrane proteins, which have the ability to uptake dsRNA into the cells. Also the cellular responses against dsRNA uptake were analyzed using silkworm cells. As a result, we found that C. elegans Sid-1 membrane protein possessed an ability to uptake dsRNA efficiently. Using this protein, several transgenic insect cell lines were successfully established. Furthermore, almost all RNAi related components of silkworm were isolated and characterized.
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DOI: 10.4161/rna.9.1.18084
发表时间: 2012-01-01
期刊: RNA BIOLOGY
影响因子: 4.1
作者: [Mon, Hiroaki, Kobayashi, Isao, Kusakabe, Takahiro]
通讯作者: Kusakabe, Takahiro
カイコ培養細胞NHEJにおける修復関連遺伝子ノックダウンの影響
修复相关基因敲除对培养家蚕细胞NHEJ的影响
DOI: --
发表时间: 2012
期刊:
影响因子: --
作者: [藤井美江, 門宏明, 高橋将晃, 光延仁志, 李在萬, 日下部宜宏]
通讯作者: 日下部宜宏
DOI: 10.1016/j.ibmb.2013.04.004
发表时间: 2013-08
期刊: Insect biochemistry and molecular biology
影响因子: 3.8
作者: [Li Zhu;Tsuneyuki Tatsuke;H. Mon;Zhiqing Li;Jian Xu;J. M. Lee;T. Kusakabe]
通讯作者: Li Zhu;Tsuneyuki Tatsuke;H. Mon;Zhiqing Li;Jian Xu;J. M. Lee;T. Kusakabe
A MC motif in silkworm Ago1 are indispensible for translational repression
家蚕 Ago1 中的 MC 基序对于翻译抑制是必不可少的
DOI: --
发表时间: 2013
期刊: Insect Mol. Biol
影响因子: --
作者: [Li Zhu, Yuuki Masaki, Tsuneyuki Tatsuke, Hiroaki Mon, Zhiqing Li, Jian Xu, JaeMan Lee, Takahiro Kusakabe]
通讯作者: Takahiro Kusakabe
19
    Analysis and modification of protein glycosylation in an insect cell expression system
    • 批准号:
      15H04612
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $10.15万
    • 财政年份:
      2015
    • 负责人:
      LEE JAEMAN
    • 依托单位:
    Establishment of the efficient dsDNA delivery system into insect cells
    • 批准号:
      26660271
    • 项目类别:
      Grant-in-Aid for Challenging Exploratory Research
    • 资助金额:
      $2.41万
    • 财政年份:
      2014
    • 负责人:
      LEE JAEMAN
    • 依托单位:
    海外基金