Analysis of dsRNA mechanism of response in insect cell

昆虫细胞中双链RNA反应机制分析

• 批准号: 23580077
• 负责人: LEE JAEMAN
• 金额: $ 3.41万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580077/
• 关键词: RNA interference; 遺伝子機能阻害; dsRNA応答機構; BmAgo1; BmAgo2; BmMLV; RISC因子; RNAi; RNAi誘導実験; siRNA; miRNA; Soaking RNAi誘導; 遺伝子機能解析; sid-1; BmN4細胞

In order to establish efficient RNA interference induction system in insect cells, we introduced several membrane proteins, which have the ability to uptake dsRNA into the cells. Also the cellular responses against dsRNA uptake were analyzed using silkworm cells. As a result, we found that C. elegans Sid-1 membrane protein possessed an ability to uptake dsRNA efficiently. Using this protein, several transgenic insect cell lines were successfully established. Furthermore, almost all RNAi related components of silkworm were isolated and characterized.

为了在昆虫细胞中建立高效的RNA干扰诱导系统，我们引入了几种具有摄取dsRNA能力的膜蛋白。还使用家蚕细胞分析了针对dsRNA摄取的细胞应答。结果表明，C.线虫Sid-1膜蛋白具有高效摄取dsRNA的能力。利用该蛋白，成功建立了几种转基因昆虫细胞系。此外，几乎所有的RNAi相关组分的家蚕被分离和鉴定。

项目成果

Effective RNA interference in cultured silkworm cells mediated by overexpression of Caenorhabditis elegans SID-1

• DOI: 10.4161/rna.9.1.18084
• 发表时间: 2012-01-01
• 期刊: RNA BIOLOGY
• 影响因子: 4.1
• 作者: Mon, Hiroaki;Kobayashi, Isao;Kusakabe, Takahiro
• 通讯作者: Kusakabe, Takahiro

カイコ培養細胞NHEJにおける修復関連遺伝子ノックダウンの影響

修复相关基因敲除对培养家蚕细胞NHEJ的影响

• 发表时间: 2012
• 作者: 藤井美江;門宏明;高橋将晃;光延仁志;李在萬;日下部宜宏
• 通讯作者: 日下部宜宏

Characterization of Tudor-sn-containing granules in the silkworm, Bombyx mori.

• DOI: 10.1016/j.ibmb.2013.04.004
• 发表时间: 2013-08
• 期刊: Insect biochemistry and molecular biology
• 影响因子: 3.8
• 作者: Li Zhu;Tsuneyuki Tatsuke;H. Mon;Zhiqing Li;Jian Xu;J. M. Lee;T. Kusakabe

A MC motif in silkworm Ago1 are indispensible for translational repression

家蚕 Ago1 中的 MC 基序对于翻译抑制是必不可少的

• 发表时间: 2013
• 期刊: Insect Mol. Biol
• 作者: Li Zhu;Yuuki Masaki;Tsuneyuki Tatsuke;Hiroaki Mon;Zhiqing Li;Jian Xu;JaeMan Lee;Takahiro Kusakabe
• 通讯作者: Takahiro Kusakabe

Role of silkworm RISC components in the basal defense against Bombyx mori nucleopolyhedrovirus (BmNPV)

家蚕 RISC 成分在家蚕核多角体病毒 (BmNPV) 基础防御中的作用

• 发表时间: 2014
• 作者: 祝 力・徐 剣・李 志清・門 宏明・ 李 在萬・日下部宜宏