Clarification of mechanism of biofilm formation and degradation of toxic compounds by genome sequencing and numerical analysis

本研究主要针对降解芳香族化合物的细菌的生物膜形成进行研究。对一株多氯联苯降解菌Comamonas testosteroni TK102的基因组进行了分析，以分析其在降解和生物膜形成过程中的特异性表达。我们还进行了生物膜生长的模拟。添加联苯羟基化合物可促进生物膜的形成和活细胞的数量。利用下一代测序系统对菌株TK102进行全基因组测序，并将其降解基因编码在一个可移动的遗传元件上。数值结果表明，生物膜种子的初始位置是一个重要的影响因素，生物膜上的流动对流和营养物质扩散到生物膜中的相对关系影响着生物膜的生长行为。

This research was focused on the biofilm formation of bacteria which degrade aromatic compounds. Genome of a PCB degrader Comamonas testosteroni TK102 was determined for the analysis of specific expression during degradation and biofilm formation. We also carried out a biofilm growth simulation. Addition of hydroxyl compound of biphenyl enhanced biofilm formation and number of live cells. Whole genome sequence of strain TK102 was done by next generation sequencing system and PCB degradation genes were coded on a mobile genetic element. From numerical results, the initial position of biofilm seeds proved to be an important factor and growth behavior was influenced by the relative relationship between convection of flow over biofilm and nutrient diffusion into biofilm.