Development of immobilized koji-mold catalyst for effective isolation of pharmaceutically significant yerba mate compounds

以马黛茶为原料，建立了一种以曲霉为催化剂制备抗流感药莽草酸的新工艺。马茶提取物对黑曲霉等菌丝体的绿原酸水解酶（CGA）有较强的诱导作用。菌丝在40˚C下热处理30 min，多酚氧化酶被破坏，但gase在热处理过程中表现出较强的抗性并存活。因此，加热后的菌丝可作为一种固定化催化剂。研究了用固定化加热菌丝作为case的模型反应。这是本研究最突出的一点，该方法可用于QA和咖啡酸（CA）的工业生产。在之前的研究中已经证实，QA可以通过乙酸细菌的两种酶体系转化为莽草酸。采用简单的离子交换柱色谱法，建立了同时分离QA、CGA和CA的方法。

A novel strategy preparing raw materials for pharmaceuticals such as shikimic acid for Tamiflu was established applying koji-mold catalyst to yerba mate tea. Chlorogenic acid (CGA) hydrolase (CGase) was potently induced in mycelia such as Aspergillus niger when grown on yerba mate extract. Heat treatment of mycelia at 40˚C for 30 min destroyed polyphenol oxidase but CGase was highly resistant and survived during the heat treatment.Thus, the heated mycelia were found useful as a kind of immobilized catalyst of CGase. Some model reaction using immobilized heated mycelia as CGase was studied. This is the most outstanding point in this study and this method can be accepted for industrial production of QA and caffeic acid (CA). QA can be converted to shikimate by two enzyme systems of acetic acid bacteria, as has already been established in the previous study. Simultaneous isolation of QA, CGA, and CA was established by a simple ion exchange column chromatography.