Search for chemoattractant of Ralstonia solanacearum in tomato root exudate

在番茄根系分泌物中寻找青枯菌化学引诱剂。将番茄根系分泌物分离为乙酸乙酯可溶性组分、吸附组分和非吸附组分，并测定了它们的化学引诱活性。通过活性炭柱的纤维素酶活性最强，吸附纤维素酶活性较弱。通过比较杂交种与等量氨基酸混合物的化学引诱活性，发现杂交种中存在非氨基酸类的化学引诱物。制备青枯菌属（solanacearum），即水稻、油菜和苜蓿，并检测它们的化学引诱活性。结果表明，番茄的活性炭柱通过率比其它植物的活性炭柱通过率高。

Chemoattractant for Ralstonia solanacearum was searched in the root exudate of tomato. The root exudate of tomato was fractionated into EtOAc-soluble fr. and absorbed and non-absorbed (passed) fr. of activated charcoal column, and their chemoattractive activity was examined. Passed fr. of activated charcoal column showed strongest activity and absorbed fr. was less active. Comparison of chemoattractive activity between passed fr. and amino acid mixture equivalent to the fr. showed the presence of chemoattractant other than amino acids.Fractionated root exudates of non-host plant of R. solanacearum, i.e. rice, rape and alfalfa were prepared and their chemoattractive activity was examined. As a result, passed fr. of activated charcoal column of tomato showed stronger activity than those of other plants.