Search for chemoattractant of Ralstonia solanacearum in tomato root exudate

从番茄根系分泌物中寻找番茄青枯病的化学引诱剂。采用活性炭柱对番茄根系分泌物进行分离，将其分为乙酸乙酯可溶性菌群、吸附菌群和未吸附（通过）菌群，考察其化学吸引活性。通过活性炭柱的活性最强，吸收的活性较弱。通过对传代菌株和与菌株相当的氨基酸混合物的化学吸引活性比较，发现菌株中存在氨基酸以外的化学吸引剂。制备了非寄主植物水稻、油菜和苜蓿的根系分泌物，并对其化学吸引活性进行了测定。结果表明，番茄通过的活性炭柱活性较其他植物强。

Chemoattractant for Ralstonia solanacearum was searched in the root exudate of tomato. The root exudate of tomato was fractionated into EtOAc-soluble fr. and absorbed and non-absorbed (passed) fr. of activated charcoal column, and their chemoattractive activity was examined. Passed fr. of activated charcoal column showed strongest activity and absorbed fr. was less active. Comparison of chemoattractive activity between passed fr. and amino acid mixture equivalent to the fr. showed the presence of chemoattractant other than amino acids.Fractionated root exudates of non-host plant of R. solanacearum, i.e. rice, rape and alfalfa were prepared and their chemoattractive activity was examined. As a result, passed fr. of activated charcoal column of tomato showed stronger activity than those of other plants.