Functional analysis of novel terpene cyclases that resemble transporter

类似转运蛋白的新型萜烯环化酶的功能分析

• 批准号: 23580153
• 负责人: KUSHIRO Tetsuo
• 金额: $ 3.49万
• 依托单位: Meiji University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580153/
• 关键词: メロテルペノイド; 糸状菌; テルペン環化酵素; ポリケタイド; 生合成

Pyripyropene, produced by a fungus Aspergillus fumigatus, is a meroterpenoid that is expected to be developed as a cholesterol lowering drug. Among the pyripyropene biosynthetic gene cluster, a novel terpene cyclase was identified that resembled more like a transporter. In this study, detailed analysis of its active site residue was carried out. Besides, a second meroterpenoid biosynthetic gene cluster was identified from A. fumigatus as well as from a fungus Fusarium graminearum, which causes Fusarium head blight. The PKS among the second gene cluster in A. fumigatus was shown to produce TAL.

Pyripyropene 由真菌 Aspergillus fumigatus 产生，是一种拟萜类化合物，有望开发为降胆固醇药物。在pyripyropene生物合成基因簇中，鉴定出一种新型萜烯环化酶，它更像转运蛋白。本研究对其活性位点残基进行了详细分析。此外，从烟曲霉和引起赤霉病的真菌禾谷镰刀菌中鉴定出了第二个类萜生物合成基因簇。烟曲霉第二个基因簇中的 PKS 被证明可产生 TAL。

项目成果

Identification of a Key Prenyltransferase Involved in Biosynthesis of the Most Abundant Fungal Meroterpenoids Derived from 3,5-Dimethylorsellinic Acid.

• DOI: 10.1002/cbic.201200124
• 发表时间: 2012-05-29
• 期刊: CHEMBIOCHEM
• 影响因子: 3.2
• 作者: Itoh, Takayuki;Tokunaga, Kinya;Kushiro, Tetsuo
• 通讯作者: Kushiro, Tetsuo