Nobiletin, a polymethoxylated flavone from citrus peels, induces differentiation of normal human epidermal keratinocytes

诺比林是从柑橘皮中提取的一种多甲氧基黄酮类化合物，具有广泛的药理活性。在这项研究中，我们研究了nobiletin对人表皮角质形成细胞分化的影响。研究了几种柑橘多甲氧基黄酮类成分对诱导分化的影响。我们目前的发现表明，B环上两个甲氧基的存在似乎是诱导分化的关键。此外，本研究结果提供了可能的证据，即ERK/CREB信号通路的激活在一定程度上介导了nobiletin对人表皮角质形成细胞分化的促进作用。此外，还评价了局部应用nobiletin对剥离屏障后表皮屏障恢复率的影响。在剥离胶带后，nobiletin立即加速了无毛小鼠皮肤的屏障恢复。

Nobiletin, a polymethoxylated flavone from citrus peels, is known to have a wide range of pharmacological activities. In this study, we examined the effects of nobiletin on differentiation of human epidermal keratinocytes. We examined the effects of several polymethoxyl flavones of Citrus on induction of differentiaon. Our present findings suggest that the presence of two methoxyl groups on the B ring appears to be critical for the differentiation-inducing effect. In addition, the results obtained here provide possible evidence that the activation of ERK/CREB signaling in part mediates improvement of differentiation of human epidermal keratinocytes induces by nobiletin.Furthermore, the effects of topical application of nobiletin on the epidermal barrier recovery rate after barrier disruption by tape stripping were evaluated. Immediately after tape stripping, nobiletin accelerated the barrier recovery on hairless mouse skin.