Functional analysis of the drought-induced LEA protein from Lombardy poplar

伦巴第杨干旱诱导的 LEA 蛋白的功能分析

• 批准号: 23580217
• 负责人: NISHIGUCHI Mitsuru
• 金额: $ 3.24万
• 依托单位: Forestry and Forest Products Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580217/
• 关键词: ポプラ; LEA; 環境ストレス; 遺伝子組換え; 耐塩性; 発現機構; 樹木; 発現誘導

To elucidate the function of LEA proteins inferred to be involved in the response to environmental stress in woody plants, the cDNAs of LEA proteins were isolated from Lombardy poplar and were investigated. Among of the poplar LEA genes, the expression of PnLEA1 was induced by drought, high salinity and low temperature. We found that PnLEA1 plays an important role for initial adaptation to salinity stress, because the expression of PnLEA1 recovered the delay in cell growth of Escherichia coli under high salinity. However, the PnLEA1-overexpressed poplar plants were withered after about 14 days without watering as well as empty vector control plants.

为了阐明推测参与木本植物环境胁迫响应的 LEA 蛋白的功能，从伦巴第杨中分离了 LEA 蛋白的 cDNA 并进行了研究。杨树LEA基因中，PnLEA1的表达受干旱、高盐和低温诱导。我们发现PnLEA1对于盐度胁迫的初始适应起着重要作用，因为PnLEA1的表达恢复了大肠杆菌在高盐度下细胞生长的延迟。然而，PnLEA1过表达的杨树植物和空载体对照植物在大约14天不浇水后枯萎。

项目成果

ポプラの乾燥誘導性LEAタンパク質遺伝子の機能解析

杨树干旱诱导LEA蛋白基因的功能分析

• 发表时间: 2012
• 作者: SAKAI Hisao;MIYAMOTO Kazuki;MORISHITA Tomoaki;NOGUCHI Kyotaro;酒井寿夫;西口満
• 通讯作者: 西口満