Development of transformation system in Lombardy poplar by using a poplar gene as a selective marker gene

为获得转基因植株的选择标记基因，本研究克隆了伦巴第白杨（Populus nigra var.意大利）。在芽殖酵母中过表达GPT赋予衣霉素耐受性。利用衣霉素和一种新的双元载体，成功地将GPT基因导入杨树，获得了转GPT基因杨树。

To obtain a selective marker gene for generation of transgenic trees, we cloned and sequenced the cDNA of UDP-N-acetylglucosamine : dolichyl-phosphate N-acetylglucosaminephosphotransferase (GPT) from Lombardy poplar (Populus nigra var. italica). Overexpression of GPT in budding yeast conferred tunicamycin tolerance. We succeeded in the generation of GPT-introduced transgenic poplars by using tunicamycin and a new binary vector, which contained the cDNA of GPT as a selective marker gene.