Development of transformation system in Lombardy poplar by using a poplar gene as a selective marker gene

为了获得转基因树的选择标记基因，我们克隆并测序了伦巴第杨UDP-N-乙酰氨基葡萄糖：N-乙酰氨基葡萄糖磷酸转移酶(GPT)的基因。意大利语)。GPT在芽殖酵母中的过表达增强了衣霉素的耐受性。利用衣霉素和以GPT基因为选择标记基因的新型双元载体，成功地获得了GPT转基因杨树。

To obtain a selective marker gene for generation of transgenic trees, we cloned and sequenced the cDNA of UDP-N-acetylglucosamine : dolichyl-phosphate N-acetylglucosaminephosphotransferase (GPT) from Lombardy poplar (Populus nigra var. italica). Overexpression of GPT in budding yeast conferred tunicamycin tolerance. We succeeded in the generation of GPT-introduced transgenic poplars by using tunicamycin and a new binary vector, which contained the cDNA of GPT as a selective marker gene.