Development of transformation system in Lombardy poplar by using a poplar gene as a selective marker gene

以杨树基因为选择标记基因开发伦巴第杨树转化系统

• 批准号: 19580178
• 负责人: NISHIGUCHI Mitsuru
• 金额: $ 2.25万
• 依托单位: Forestry and Forest Products Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19580178/
• 关键词: ポプラ; 遺伝子組換え; 選抜マーカー遺伝子; ツニカマイシン; GPT; バイナリーベクター; 遺伝子発現; 出芽酵母; 選抜マーカー

To obtain a selective marker gene for generation of transgenic trees, we cloned and sequenced the cDNA of UDP-N-acetylglucosamine : dolichyl-phosphate N-acetylglucosaminephosphotransferase (GPT) from Lombardy poplar (Populus nigra var. italica). Overexpression of GPT in budding yeast conferred tunicamycin tolerance. We succeeded in the generation of GPT-introduced transgenic poplars by using tunicamycin and a new binary vector, which contained the cDNA of GPT as a selective marker gene.

为获得转基因植株的选择标记基因，本研究克隆了伦巴第白杨（Populus nigra var.意大利）。在芽殖酵母中过表达GPT赋予衣霉素耐受性。我们利用衣霉素和一种新的双元载体成功地获得了转GPT基因杨树，该双元载体含有GPT作为选择标记基因的cDNA。