Basic studies on development of a novel delivery system of materials into fish oocytes

鱼类卵母细胞新型材料输送系统开发的基础研究

• 批准号: 23580243
• 负责人: HIRAMATSU Naoshi
• 金额: $ 3.49万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580243/
• 关键词: 魚類; 受容体標的輸送; ビテロジェニン; ビテロジェニン受容体; 蛋白質相互作用; 卵母細胞

A yolk precursor vitellogenin (Vg) is selectively incorporated from the maternal blood into oocyte following its binding to ovarian Vg receptor. This study demonstrated that Vg obtained from heterologous species, as well as Vg coupled with voluntary lipids, can be delivered to fish eggs and offsprings by injecting them into broodstocks. This indicated a potential use of fish Vg as a transporter of bioactive materials; the system can be universal to any fish species. This study also confirmed that such system was found to be applicable for the delivery of bioactive lipids and/or hydrophobic materials to eggs and offsprings across generations.

卵黄前体卵黄蛋白原（Vg）与卵巢Vg受体结合后，从母体血液中选择性地掺入卵母细胞。这项研究表明，从异源物种获得的Vg，以及Vg与自愿脂质耦合，可以通过注射到亲鱼卵和后代。这表明鱼类Vg作为生物活性物质转运蛋白的潜在用途;该系统可以通用于任何鱼类。该研究还证实，发现这种系统适用于跨代向卵和后代递送生物活性脂质和/或疏水材料。

项目成果

Dual vitellogenins in cutthroat trout (Oncorhynchus clarki) : purification and changes in serum protein level during reproductive cycle

鲤鱼（Oncorhynchus clarki）中的双卵黄原蛋白：繁殖周期中血清蛋白水平的纯化和变化

• 发表时间: 2012
• 作者: 鷲尾洋平;横井勇人;鈴木徹;Mushirobira Y. 他8名
• 通讯作者: Mushirobira Y. 他8名

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Ligand binding properties of ovarian lipoprotein receptors in the cutthroat trout

鳟鱼卵巢脂蛋白受体的配体结合特性

• 发表时间: 2014
• 作者: Mushirobira;Y.;Mizuta;H.;Luo;W.;Todo;T.;Hara;A.;Reading B.J.;Sullivan;C.V. and Hiramatsu N.
• 通讯作者: C.V. and Hiramatsu N.

Ovarian yolk formation in fishes: Molecular mechanisms underlying formation of lipid droplets and vitellogenin-derived yolk proteins

• DOI: 10.1016/j.ygcen.2015.01.025
• 发表时间: 2015-09-15
• 期刊: GENERAL AND COMPARATIVE ENDOCRINOLOGY
• 影响因子: 2.7
• 作者: Hiramatsu, Naoshi;Todo, Takashi;Hara, Akihiko
• 通讯作者: Hara, Akihiko

卵黄蛋白前駆物質ビテロジェニンの異種間投与とその運搬過程:イトウとゼブラフィッシュを用いたモデルについて

蛋黄蛋白前体卵黄蛋白原及其转运过程的跨物种管理：关于使用 itou 和斑马鱼的模型

• 发表时间: 2011
• 作者: Mushirobira Y.;Mizuta H.;Luo W.;Morita Y.;Sawaguchi S.;Matsubara T.;Hiramatsu N.;Todo T.;Hara A.;西宮攻・勝義直・稲川裕之・平松尚志・東藤孝・原彰彦;櫻井秀之・川北奈央子・平松尚志・東藤孝・原彰彦
• 通讯作者: 櫻井秀之・川北奈央子・平松尚志・東藤孝・原彰彦