Development of non-destructive techniques for determining of rice amylose content by combined use of near-infrared spectroscopy and visible light imaging

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A calibration model was developed by combined use of the data of amylose content predicted by the NIR spectrometer and color information obtained by the VIS segregator (NIR&VIS) by using multiple linear regression method (dual-step calibration method). The validation statistics between reference amylose content and NIR&VIS-predicted amylose content indicated that the calibration model can be used for screening of rice sample according to amylose content.The validation rice sample set was isolated from calibration rice sample set in this study. The validation set included rice samples of next year products compared to the calibration set. This is the same condition in practical use of this non-destructive method at grain elevators. The results indicated that the combined use of an NIR spectrometer and a VIS segregator enables reasonable non-destructive determination of amylose content of rice at grain elevators.