Development of efficient gene markers that can be used to improve the fat deposition traits in pigs.

开发可用于改善猪脂肪沉积性状的有效基因标记。

• 批准号: 23580393
• 负责人: TANAKA Kazuaki
• 金额: $ 3.16万
• 依托单位: Azabu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580393/
• 关键词: ブタ; 改良; 遺伝子マーカー; 脂肪蓄積; グレリン; グレリン受容体; レプチン受容体; Gタンパク質共役型受容体120; グレリン遺伝子; グレリン受容体遺伝子; レプチン受容体遺伝子; 相関解析; 経済形質; 遺伝子多型マーカー; GPR120遺伝子; 遺伝子多型

In the present study, we analyzed sequence variations in pig homolog of candidate genes for human obesity in order to develop efficient gene markers that can be used to improve the fat deposition traits in pigs. The preproghrelin (GHRL), growth hormone secretagogue receptor (GHSR), leptin receptor (LEPR), and G-protein coupled receptor 120 (GPR120) genes were selected to study. The c.335C>A polymorphism in GHRL gene was not associated with intra-muscular fat and back fat thickness. However, previously reported c.2002C>T (now c.1987C>T) polymorphism in LEPR was significantly associated with back fat thickness in our study population, and thus, we confirmed that this polymorphism could be an effective gene marker. Furthermore, we have succeeded in developing a simple genotyping method for this polymorphism.

在本研究中，我们分析了人类肥胖候选基因的猪同源序列变异，以开发有效的基因标记，可用于改善猪的脂肪沉积性状。选择前促生长素（GHRL）、生长激素促分泌受体（GHSR）、瘦素受体（LEPR）和g蛋白偶联受体120 （GPR120）基因进行研究。GHRL基因c.335C . >多态性与肌内脂肪和背部脂肪厚度无关。然而，先前报道的LEPR中c.2002C>T（现为c.1987C>T）多态性与我们研究人群的背部脂肪厚度显著相关，因此，我们证实该多态性可能是一个有效的基因标记。此外，我们已经成功地开发了一种简单的基因分型方法。

项目成果

Evaluation of effects of multiple candidate genes (LEP, LEPR, MC4R, PIK3C3, and VRTN) on production traits in Duroc pigs

• DOI: 10.1111/asj.12134
• 发表时间: 2014-03-01
• 期刊: ANIMAL SCIENCE JOURNAL
• 影响因子: 2
• 作者: Hirose, Kensuke;Ito, Tetsuya;Tanaka, Kazuaki
• 通讯作者: Tanaka, Kazuaki