Identification of lesions responsible for fatal outcome in rats infected with Borna disease virus

新生大鼠颅内接种博尔纳病病毒（Borna disease virus，BDV）的CRNP 5变异株、CRP3 BDV变异株和9种重组BDV变异株（rBDV），观察8周。1. CRNP 5在博尔纳病（BD）的发病率和严重程度方面得分最高。四组rBDV变体的评分高于CRP 3。 2.从接种后第20天开始，4个rBDV变异体的体重增长低于其它rBDV变异体和CRP 3。3.所有接种BDV的脑组织均呈抗BDV抗体阳性。幼仔死亡的病毒滴度显著高于处死时的幼仔。4.包括L1（L1417R）突变在内的4个rBDV变异株的单核细胞血管周围套囊严重程度较高，L1（L1417R）突变可能在BD的致病性中起重要作用。5.仅在rBDV变体中观察到脑膜脑炎。 幼犬死亡率得分较高。

Newborn rats were inoculated intracranially with CRNP5 variant of Borna disease virus (BDV) with severe pathogenesis, CRP3 BDV variant with mild pathogenesis, and nine recombinant BDV (rBDV) variants and examined for 8 weeks. 1. The CRNP5 had the highest score for incidence and severity of Borna disease (BD). Four groups of rBDV variants had a higher score than CRP3. 2. The body weight increase of four rBDV variants was lower than that of other rBDV variants and CRP3 from day 20 after inoculation. 3. All BDV inoculated brains were positive for anti BDV antibodies immunohistochemically. The virus titer for pup fatalities was significantly higher than that of pups on termination. 4. Four rBDV variants including L1(L1417R) mutation had higher scores for the severity of perivascular cuffing of mononuclear cells and L1(L1417R) may play an important role for the pathogenicity of BD. 5. Menigoencephalitis was observed only in rBDV variants. The pup fatalities had higher scores.