The role of vector saliva in transmission of vector-borne diseases

媒介唾液在媒介传播疾病传播中的作用

• 批准号: 23580424
• 负责人: KATO HIROTOMO
• 金额: $ 3.41万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580424/
• 关键词: サシチョウバエ; 唾液; リーシュマニア; 生理活性物質; ベクター

The salivary gland transcriptome analysis of Lutzomyia ayacuchensis, a vector of cutaneous leishmaniasis in Ecuadorian Andes, was performed and abundant transcripts coding for secreted proteins were identified. The most abundant transcript coded for an RGD-containing peptide was expressed in Escherichia coli and its activity was characterized. The resulting protein inhibited both platelet aggregation and blood coagulation pathway, indicating that the peptide plays an important role in the blood feeding of Lu. ayacuchensis by inhibiting host hemostasis via dual mechanisms. On the other hand, salivary nucleotidases of sand flies were assessed on their activity as an exacerbation factor for Leishmania infection. Salivary nucleotidases, PduApy and PduADA, slightly accelerated the disease progression after co-injection of Leishmania parasites, suggesting that these proteins in saliva may be partly associated with exacerbation of the disease in leishmaniasis.

对厄瓜多尔安第斯山脉中皮肤利什曼病的向量的lutzomyia ayacuchensis的唾液腺转录组分析进行了，并确定了编码分泌蛋白质的丰富转录本。编码为含RGD的肽的最丰富的转录本在大肠杆菌中表达，其活性的表征。所得的蛋白质抑制了血小板聚集和血液凝结途径，表明该肽在LU的血液进食中起重要作用。通过双重机制抑制宿主止血，阿扬孔感。另一方面，评估了沙蝇的唾液核苷酸酶，以作为利什曼原虫感染的恶化因子的活性评估。唾液核苷酸酶，PDUAPY和PDUADA在共同注射利什曼原虫寄生虫后略微加速了疾病的进展，这表明唾液中的这些蛋白质可能部分与利什曼病中疾病加剧的疾病有关。

项目成果

Leishmaniasis recidiva cuitis and its topical treatment in Ecuador

厄瓜多尔利什曼病常发性皮肤炎及其局部治疗

• 发表时间: 2013
• 期刊: Trop Med Health
• 影响因子: 4.5
• 作者: Calvopiña M;Kato H;Hashiguchi Y.
• 通讯作者: Hashiguchi Y.

Transcriptome analysis of salivary glands of Lutzomyia ayacuchensis, a vector of leishmaniasis

利什曼病载体 Lutzomyia ayacuchensis 唾液腺的转录组分析

• 发表时间: 2011
• 期刊: 獣医寄生虫学会誌
• 作者: Kato H;Gomez EA;Jochim RC;Uezato H;Valenzuela JG;Hashiguchi Y.
• 通讯作者: Hashiguchi Y.

Analysis of salivary gland transcripts of the sand fly Lutzomyia ayacuchensis, a vector of Andean-type cutaneous leishmaniasis

• DOI: 10.1016/j.meegid.2012.08.024
• 发表时间: 2013-01-01
• 期刊: INFECTION GENETICS AND EVOLUTION
• 影响因子: 3.2
• 作者: Kato, Hirotomo;Jochim, Ryan C.;Hashiguchi, Yoshihisa
• 通讯作者: Hashiguchi, Yoshihisa

2つの作用機序で止血機構を阻害するサシチョウバエ唾液腺由来RGDペプチドayadualin

Ayadualin，一种源自白蛉唾液腺的 RGD 肽，通过两种作用机制抑制止血

• 发表时间: 2014
• 作者: 片倉賢;Alam Mohammad;中尾亮;櫻井達也;加藤大智;Chang K-P.;加藤大智,Eduardo A. Gomez,藤田恵,石丸由佳,橋口義久
• 通讯作者: 加藤大智,Eduardo A. Gomez,藤田恵,石丸由佳,橋口義久

Distribution of causative agents (Leishmania spp.) and sand fly vectors (Lutzomyia spp.) of cutaneous and mucocutaneous leishmaniasis in Ecuador

厄瓜多尔皮肤和粘膜利什曼病病原体（利什曼原虫属）和白蛉媒介（Lutzomyia spp.）的分布

• 发表时间: 2013
• 作者: Hirotomo Kato;Eduardo Gomez L.;Luiggi Martini R.;Jenny Muzzio Aroca;Manuel Calvopina H. and Yoshihisa Hashiguchi
• 通讯作者: Manuel Calvopina H. and Yoshihisa Hashiguchi