Study on molecular basis of growth factor/receptor of canine hemangiosarcoma

犬血管肉瘤生长因子/受体的分子基础研究

• 批准号: 23580439
• 负责人: SAKAI Hiroki
• 金额: $ 3.24万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580439/
• 关键词: 血管肉腫; 犬; 培養細胞株; mTOR; PDGFR; DNAアレイ; PDGF受容体; 遺伝子変異; MUC-1; 株化細胞; ｍTOR; Akt; Erk; シグナル伝達系; 増殖因子; ヌードマウス; 移植; 細胞株

Seven canine hemangiosarcoma cell lines were established for analysis of pathogenesis of hemangiosarcoma. All cell lines showed active intake of DiI-Ac-LDL, and positive for CD31, VEGF-A, bFGF, HGF, IGF-I and PDGF-B mRNA by RT-PCR. Constitutive activation of mTORC2/Akt/4E-BP1 associated with the proliferation of cell lines in vitro and in vivo, and this activation was confirmed in spontaneous canine hemangiosarcomas. Moreover, the high expression of PDGF receptor beta without genomic mutation of jaxutamembrane area was detected in hemangiosarcoma. It is suggested that mTOR and PDGF receptor beta may be considered as one of therapeutic targets.

建立了7个犬血管肉瘤细胞系，用于分析血管肉瘤的发病机制。RT-PCR结果显示，所有细胞株均表达CD31、VEGF-A、bFGF、HGF、IGF-I和PDGF-BmRNA。MTORC2/Akt/4E-BP1的结构性激活与体内外细胞的增殖有关，并在自发性犬血管肉瘤中得到证实。此外，在血管肉瘤中还检测到无基因组突变的巨噬细胞膜区PDGF受体β的高表达。提示mTOR和PDGF受体β可作为治疗的靶点之一。

项目成果

坐骨神経に発生した組織球肉腫の犬の一例

犬坐骨神经组织细胞肉瘤一例

• 发表时间: 2013
• 作者: 13.淺田 慎也;高村 文子;吉田 俊一;酒井 洋樹;前田 貞俊;神志那 弘明
• 通讯作者: 神志那 弘明

Expression of platelet-derived growth factor and its receptors in spontaneous canine hemangiosarcoma and cutaneous hemangioma

• DOI: 10.14670/hh-27.601
• 发表时间: 2012-05-01
• 期刊: HISTOLOGY AND HISTOPATHOLOGY
• 影响因子: 2
• 作者: Abou Asa, S.;Murai, A.;Sakai, H.
• 通讯作者: Sakai, H.

Ganglioneuroma in the Urinary Bladder of a Dog

• DOI: 10.1292/jvms.10-0523
• 发表时间: 2011-06-01
• 期刊: JOURNAL OF VETERINARY MEDICAL SCIENCE
• 影响因子: 1.2
• 作者: Sakai, Hiroki;Yonemaru, Kayoko;Yanai, Tokuma
• 通讯作者: Yanai, Tokuma

Multimodal therapy for the treatment of an oncocytoma in the nasal cavity of a cat.

用于治疗猫鼻腔嗜酸细胞瘤的多模式疗法。

• DOI: 10.1111/jsap.12094
• 发表时间: 2013
• 期刊: J Small Anim Pract
• 影响因子: 1.6
• 作者: Mori T;Mishima H;Sakai H;Noguchi S;Maruo K.
• 通讯作者: Maruo K.

MRIによりメラノーマと誤診した虹彩血管肉腫の一例

虹膜血管肉瘤MRI误诊为黑色素瘤一例

• 发表时间: 2013
• 作者: Fushimi Y;Takagi M;Hasunuma H;Uno S;Kokushi E;Watanabe U;Liu J;Marey MA;Miyamoto A;Otoi T;Deguchi E;Fink-Gremmels J.;村上麻美,森崇,伊藤祐典,酒井洋樹,山瀬新悟,川部美史,丸尾幸嗣
• 通讯作者: 村上麻美,森崇,伊藤祐典,酒井洋樹,山瀬新悟,川部美史,丸尾幸嗣