Study on molecular basis of growth factor/receptor of canine hemangiosarcoma

点击翻译按钮获取中文摘要

Seven canine hemangiosarcoma cell lines were established for analysis of pathogenesis of hemangiosarcoma. All cell lines showed active intake of DiI-Ac-LDL, and positive for CD31, VEGF-A, bFGF, HGF, IGF-I and PDGF-B mRNA by RT-PCR. Constitutive activation of mTORC2/Akt/4E-BP1 associated with the proliferation of cell lines in vitro and in vivo, and this activation was confirmed in spontaneous canine hemangiosarcomas. Moreover, the high expression of PDGF receptor beta without genomic mutation of jaxutamembrane area was detected in hemangiosarcoma. It is suggested that mTOR and PDGF receptor beta may be considered as one of therapeutic targets.