Development of microbial factory for bioplastic production adapting inedible biomass hydrolysates

木质纤维生物质主要由葡萄糖和木糖组成。利用葡萄糖生产生物聚合物已被证明是有效的，而使用木糖是低效的。然而，这两种糖的有效利用是必不可少的。在这项研究中，开发和改进了有效利用木糖的方法，以获得高产量的生物聚合物。对于P(LA-co-3HB)的产生，细胞内添加乳酸有助于提高木糖的聚合物产量。此外，木糖比葡萄糖在共聚物中提供更高的LA含量。作为半乳糖转运体的GATC对木糖的吸收也是合成共聚物的有效途径。与木质纤维素衍生的混合糖相结合的高产生物聚合物的方法具有建立用于生产各种生物聚合物的生物合成的潜力。

Lignocellulosic biomass is mainly composed of glucose and xylose as the major sugars. The use of glucose for the biopolymer production has been demonstrated to be efficient whereas, the use of xylose is inefficient. However efficient utilization of both sugars is essential. In this study, methods for the efficient utilization of xylose were developed and enhanced to obtain high productivities of biopolymers. For P(LA-co-3HB) production lactic acid supplementation into the cell contributed to the inprovement of polymer yield from xylose. In addition xylose was found to be superiorb to glucose in giving higher LA fraction in the copolymer. The uptake of xylose by GatC which is galactitol transporter also effective to produce the copolymer. The approach for high yield production of biopolymers in combination with the lignocellulosic-derived mix sugars have a potential for the establishment of biorifinary for the production of variety of biopolymers.