Structural analyses of the high molecular mass peptidases belonging to M1 and S9 families

点击翻译按钮获取中文摘要

High molecular mass is a characteristic feature of the M1 and S9 family enzymes. Enzymes belonging to M1 family contain many aminopeptidases abundant in brain and those to S9 family contain oligopeptidase B, which is involved in the infection process of trypanosomiasis. These enzymes posess unique structures covering the catalytic center of cleavage activity. In this study, two peptidases, a puromycin-sensitive aminopeptidase from human and oligopeptidase B from some bacteria were used to investigate important amino acid residues for activity. In addition, crystallization conditions for some enzymes were found. It is important to understand phisiological functions of brain aminopeptidases and to develop a new way of chemotherapy against some infectious diseases.