Synergistic Effects of Calcineurin Inhibitors and Steroids on Steroid Sensitivity of Peripheral Blood Mononuclear Cells

类固醇受体（SR）复合物含有FKBP 51和FKBP 52，它们与他克莫司（TAC）和亲环素40结合，亲环素40又与环孢菌素（CYA）结合;这些影响类固醇-SR复合物的核内移动性。认为类固醇和钙调磷酸酶抑制剂（CNI）对SR复合物存在药效学相互作用。我们研究了CNIs对类固醇敏感性的影响。本文检测了30例健康人甲泼尼龙（MPSL）与CNI混合培养的激素敏感性，并对23例肾移植受者CNI治疗前后MPSL和皮质醇敏感性进行了检测。此外，TAC对增加敏感性有更大的作用。因此，CNI和类固醇的伴随施用可增加类固醇敏感性。然而，我们无法证明作用机制，尽管我们正在解决作用机制，使用Jurkat细胞敲低FKBP 51与SiRNA

The steroid receptor (SR) complex contains FKBP51 and FKBP52, which bind to tacrolimus (TAC) and cyclophilin 40, which in turn binds to cyclosporine (CYA); these influence the intranuclear mobility of steroid-SR complexes. Pharmacodynamic interactions are thought to exist between steroids and calcineurin inhibitors (CNIs) on the SR complex. We examined the effect of CNIs on steroid sensitivity. We examined the steroid sensitivity of mixed culture with both methylprednisolone (MPSL) and CNIs in 30 healthy subjects.and MPSL and cortisol sensitivity were examined before and after CNI administration in 23 renal transplant recipients.Steroid sensitivity increased with both TAC and CYA. Furthermore, TAC had a greater effect on increasing sensitivity. Thus, concomitant administration of CNIs and steroid can increase steroid sensitivity. However, we could not have demonstrated the action mechanism, though we are resolving the action mechanism, using Jurkat cell knockdown FKBP51 with SiRNA