Molecular mechanism of cellular interaction between placode and neural crest

颅外周神经系统的独特之处在于它来源于外胚层基板和神经嵴细胞（NCCs）。在发育过程中，基板与ncc之间存在着相互依存的关系。考虑到理解基板和NCCs之间相互作用的重要性，目前还没有完全揭示控制它们相互作用的配体和受体，特别是在哺乳动物中。这项研究的目的是确定相互作用的分子。我们已经报道了转录因子编码基因Six1和Six4仅在基板中表达，而不在ncc中表达。靶向这些基因的敲除小鼠胚胎不仅在胎盘上出现缺陷，而且在NCCs衍生器官上也出现缺陷。使用靶向小鼠和野生型1的基因，我们在这里寻找了基板和NCCs相互作用所必需的候选分子。我们还研究了在发育过程中抑制候选分子表达的影响。

The cranial peripheral nervous system is unique in the sense that it is derived from both ectodermal placodes and neural crest cells (NCCs). The interdependent relationship exists between placodes and NCCs all through development. Considering obvious importance of understanding the interactions between placodes and NCCs, it is not fully uncovered about the ligands and receptors governing their interactions, especially in the mammals. The aim of this research was to identify molecules for the interaction. We have already reported that transcription factor encoding genes, Six1 and Six4 expressed solely in the placodes, but not in the NCCs. Knockout mouse embryos targeting these genes showed defects not only placodal, but also NCCs derived organs. Using the gene targeting mice and wild type one, here we searched candidate molecules essential for the interaction between placodes and NCCs. We also examined the effects of inhibiting the expression of candidate molecules during development.