Effect of FAEEs on intracellular signalling

为了研究游离脂肪酸乙酯（FAEEs）是否能诱导细胞损伤，我们观察了腹膜细胞，胰腺腺泡细胞和离体血管标本的细胞内[Ca 2 +]动态。棕榈油酸乙酯可引起部分腹膜细胞[Ca ~（2+）]i轻度增加，油酸乙酯可引起胰腺腺泡[Ca ~（2+）]i增加。在胰腺标本中，FAEEs灌注后死细胞数量增加，但这种增加无法得到统计学验证。FAEE溶解于DMSO中，然而溶剂可诱导细胞损伤。总之，在实验中，我们没有检测到FAEE对细胞内信号传导的影响的任何证据。

To investigate whether free fatty acid ethylesters (FAEEs) can induce cell damage, we observed intracellular [Ca2+] dynamics in peritoneal cells, pancreatic acinar cells and isolated vascular specimens. Palmitoleic acid ethyl ester might induce slight invrease of [Ca2+]i in some peritoneal cells, and Oleic acid ethyl wster caused [Ca2+]i increase in pancreatic acinus. In pacreatic specimens, dead cells increase in number after FAEEs-perfusion, however the increase cannot statistically verified. FAEEs were dissolved in DMSO, however the solvent can induce cell damage. In conclusion, in the experiment, we cannot detect any evidences of FAEEs' effect on intracellular signalling.