Changes of Ca^2±dependent intracellular signal transduction during cell cycle

细胞周期中Ca^2±依赖性细胞内信号转导的变化

• 批准号: 15590169
• 负责人: SATOH Yoh-ichi
• 金额: $ 2.3万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590169/
• 关键词: Intracellular signaling; Intracellular calcium ion concentration; Laser confocal microscopy; Artificial epidermis; Urothelium; Oviduct epithelium; Vomeronasal organ; ATP; 細胞内カルシウムイオン; 上皮組織; 共焦点レーザー顕微鏡; 画像解析; 生組織・細胞標本

This project aimed to reveal whether cell cycle and cell maturation stage affect on intracellular calcium ion-dependent signaling system. To this end we would observe epithelial tissues which were composed of various cells being different stages.・Effect of laser radiation may disturb intracellular signaling, therefore at first, we tested phtodamage in the Ca^<2+> imaging. We found that free radical monitoring is an useful tool for photodamage, and by the monitoring, we decided suitable laser power for the imaging.・However, the confocal microscope for the imaging broke down, and the objective lens was polluted and therefore about 1 year, the experiment was delayed.・Artificial epidermis and cornea composed of various cell layers and urothelium did not respond in [Ca^<2+>]_i changes to ATP.・Sexual cycle affect on [Ca^<2+>]_i changes in oviduct epithelium of golden hamsters.・Epithelium of vomeronasal organ (Jacobson's organ) of male rats showed [Ca^<2+>]_i change response to the urine from proestrous female rats, but not to urine from gestational rats.・We concluded that the cell maturation stage affect on Ca^<2+> -dependent signaling system by [Ca^<2+>]_i changes.

本项目旨在揭示细胞周期和细胞成熟阶段是否影响细胞内钙离子依赖性信号系统。为此，我们将观察由处于不同阶段的各种细胞组成的上皮组织。激光辐射的作用可能会干扰细胞内信号传导，因此我们首先在Ca^<2+>成像中测试光损伤。我们发现自由基监测是光损伤的有用工具，通过监测，我们确定了合适的成像激光功率。·然而，用于成像的共聚焦显微镜发生故障，物镜透镜受到污染，因此实验延迟了约1年。·人工表皮、由不同细胞层组成的角膜和尿道上皮对ATP引起的[Ca^<2+>] i变化无反应。性周期对金黄地鼠输卵管上皮[Ca^<2+>] i变化的影响雄性大鼠犁鼻器（Jacobson's器）上皮细胞对动情前期雌性大鼠尿液有[Ca^<2+>] i变化反应，而对妊娠期大鼠尿液无反应。结论：细胞成熟阶段通过[Ca^<2 +>] i的变化对Ca^<2 +>依赖性信号系统产生影响。

项目成果

Response to ATP is accompanied by Ca^<2+> influx via P2X purinoceptors in the coronary arterioles of golden hamsters.

对ATP的反应伴随着Ca 2+ 通过金仓鼠冠状动脉中的P2X嘌呤受体的流入。

• 发表时间: 2004
• 期刊: Arch Histol Cytol 67
• 作者: Matsuura M;Saino T;Satoh Y
• 通讯作者: Satoh Y

Cui ZJ, Zhou YD, Satoh Y, Habara Y: "A physiological role for protoporphyrin IX photodynamic action in the rat Harderian gland?"Acta Physiol Scand. 179. 149-154 (2003)

Cui ZJ, Zhou YD, Satoh Y, Habara Y：“原卟啉 IX 光动力作用在大鼠哈德腺中的生理作用？”Acta Physiol Scand。

Response to ATP is accompanied by Ca^<2+> influx via P2X purinoceptors in the coronary arterioles of golden hamsters

对 ATP 的反应伴随着 Ca^2 通过金仓鼠冠状动脉中 P2X 嘌呤受体的流入

• 发表时间: 2004
• 期刊: Arch Histol Cytol 67
• 作者: Matsuura M;Saino T;Satoh Y
• 通讯作者: Satoh Y

Application of real-time confocal laser scanning microscopy to observe living cells in tissue specimens

应用实时共焦激光扫描显微镜观察组织标本中的活细胞

• 发表时间: 2004
• 期刊: J Electron Microsc 33
• 作者: Saino T;Satoh Y
• 通讯作者: Satoh Y

A physiological role for protoporphyrin IX photodynamic action in the rat Harderian gland?

• DOI: 10.1046/j.1365-201x.2003.01177.x
• 发表时间: 2003-10
• 期刊: Acta physiologica Scandinavica
• 作者: Z. Cui;Y. D. Zhou;Y. Satoh;Y. Habara