Changes of Ca^2±dependent intracellular signal transduction during cell cycle

本项目旨在揭示细胞周期和细胞成熟阶段对内部钙离子依赖信号系统的影响。到这个结束，我们将观察到基本问题，这些问题是由不同的阶段组成的。激光辐射可能会干扰内信号，因此，首先，我们已经在Ca^<2+>成像中测试了phodamage。我们发现了一个免费的基础监测是一个有用的光损伤工具，而通过监测，我们已经确定了成像的合适激光器能力。However，一个用于成像破裂的模糊显微镜，而目标镜头被倾斜，因此大约一年后，实验被延迟了。各种细胞层和urothelium对[Ca^<2+>]_i的改变不起作用。性循环对[Ca^<2+>]_i的影响在金锤的半导体表皮中发生了变化。雄性老鼠展示了[Ca^<2+>]中的流行性器官（Jacobson's Organ）的外延_我对前列腺女老鼠的尿液的反应，但不是对雌性老鼠的尿液的反应。We concluded that the cell maturation stage affect on Ca^<2+\-dependent signaling system by [Ca^<2+\]_i changes。

This project aimed to reveal whether cell cycle and cell maturation stage affect on intracellular calcium ion-dependent signaling system. To this end we would observe epithelial tissues which were composed of various cells being different stages.・Effect of laser radiation may disturb intracellular signaling, therefore at first, we tested phtodamage in the Ca^<2+> imaging. We found that free radical monitoring is an useful tool for photodamage, and by the monitoring, we decided suitable laser power for the imaging.・However, the confocal microscope for the imaging broke down, and the objective lens was polluted and therefore about 1 year, the experiment was delayed.・Artificial epidermis and cornea composed of various cell layers and urothelium did not respond in [Ca^<2+>]_i changes to ATP.・Sexual cycle affect on [Ca^<2+>]_i changes in oviduct epithelium of golden hamsters.・Epithelium of vomeronasal organ (Jacobson's organ) of male rats showed [Ca^<2+>]_i change response to the urine from proestrous female rats, but not to urine from gestational rats.・We concluded that the cell maturation stage affect on Ca^<2+> -dependent signaling system by [Ca^<2+>]_i changes.