Pathophysiological role of mast cells on the bone homeostasis.

肥大细胞对骨稳态的病理生理作用。

• 批准号: 23590302
• 负责人: SHIOTA Naotaka
• 金额: $ 2万
• 依托单位: Shimane University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590302/
• 关键词: 関節リウマチ; 肥満細胞; 慢性炎症; 骨粗鬆症

The number of mast cells was markedly increased in the inflamed paws of collagen-induced arthritis in mice, and mast cells were one of the major cells producing TNF-alpha. TNF-alpha positive mast cells were present extensively throughout the inflamed synovium of arthritic mice, and some of the mast cells were in close proximity of osteoclasts in the area of marked bone and cartilage destruction. Treatment with a mast cell membrane stabilizer for 8 weeks and chymase inhibitor for 4 weeks significantly reduced clinical and X-ray scores of collagen-induced arthritis, and decreased the number of TNF-alpha positive mast cells and the mRNA levels of TNF-alpha in the inflamed paws of arthritic mice. The number of osteoclasts was also decreased by treatment with a mast cell membrane stabilizer and chymase inhibitor. These observations suggest that mast cells may be involved in bone metabolism and pathogenesis of collagen-induced arthritis in mice.

在胶原性关节炎小鼠炎症足爪中，肥大细胞数量明显增多，是产生肿瘤坏死因子-α的主要细胞之一。肿瘤坏死因子-α阳性的肥大细胞广泛存在于关节炎小鼠的滑膜组织中，部分肥大细胞与破骨细胞关系密切，位于骨质和软骨破坏明显的区域。肥大细胞膜稳定剂治疗8周和糜酶抑制剂治疗4周可显著降低胶原性关节炎小鼠的临床和X线评分，减少炎症足爪中肿瘤坏死因子-α阳性的肥大细胞数和肿瘤坏死因子-α的mRNA水平。用肥大细胞膜稳定剂和糜酶抑制剂处理后破骨细胞的数量也减少了。这些观察表明，肥大细胞可能参与了小鼠的骨代谢和胶原性关节炎的发病机制。

项目成果

Pathophysiological role of mast cells in collagen-induced arthritis in mice

肥大细胞在胶原诱导的小鼠关节炎中的病理生理作用

• 发表时间: 2012
• 作者: 塩田直孝;新堀智子;奥西秀樹
• 通讯作者: 奥西秀樹

コラーゲン誘発関節炎マウスにおける肥満細胞の病態生理学的役割の解析

肥大细胞在胶原诱导关节炎小鼠中的病理生理作用分析

• 发表时间: 2012
• 作者: Naotaka Shiota;Chiko Shimbori;Hideki Okunishi
• 通讯作者: Hideki Okunishi