Is phosphorylated caspase 9 a new marker of mitotic catastrophe?

最初，我们研究了磷酸化的caspase 9是否是有丝分裂灾难的新标记物。然而，这一点很难证明。接下来，我们用免疫组织化学方法检测Survivin和Smac/DIABLO在胃癌和结直肠癌中的表达。我们观察到，在胃癌和结直肠癌中，Survivin和Smac/Diablo的表达水平不同；结直肠癌中这两种表达均显著高于胃癌。此外，我们还发现Smac/DIABLO和Survivin在高到中等分化的结直肠腺癌中的表达之间存在相关性。基于这些结果，我们认为Smac/DIABLO在结直肠癌中的表达可能上调，从而抑制Survivin的抗凋亡作用。

Initially, we examined whether phosphorylated caspase 9 is a new marker of mitotic catastrophe. However, this was difficult to prove. Next, we investigated the expression rates of survivin and Smac/DIABLO in gastric and colorectal carcinomas by immunohistochemistry. We observed that gastric and colorectal carcinomas differ in their levels of survivin and Smac/DIABLO expression; both expressions were significantly higher in colorectal carcinoma than in gastric carcinoma. Additionally, we showed that there is a correlation between the expression of Smac/DIABLO and survivin in well-to-moderately differentiated colorectal adenocarcinomas. On the basis of these results, we consider that the expression of Smac/DIABLO in colorectal carcinoma may be upregulated to suppress the anti-apoptotic effect of survivin.