Is phosphorylated caspase 9 a new marker of mitotic catastrophe?

磷酸化 caspase 9 是有丝分裂灾难的新标记吗？

• 批准号: 23590396
• 负责人: SHINTANI Michiko
• 金额: $ 3.58万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590396/
• 关键词: 胃癌; 大腸癌; リン酸化カスパーゼ9; サバイビン; Smac/DIABLO; 細胞死; 免疫組織化学; survivin; apoptosis; Autophagy; immunohistochemistry; G2/M期; 細胞周期; リン酸化caspase-9; mitotic catastrophe; 蛍光多重染色; autophagy; proximity ligation assay; 蛍光多重染色法

Initially, we examined whether phosphorylated caspase 9 is a new marker of mitotic catastrophe. However, this was difficult to prove. Next, we investigated the expression rates of survivin and Smac/DIABLO in gastric and colorectal carcinomas by immunohistochemistry. We observed that gastric and colorectal carcinomas differ in their levels of survivin and Smac/DIABLO expression; both expressions were significantly higher in colorectal carcinoma than in gastric carcinoma. Additionally, we showed that there is a correlation between the expression of Smac/DIABLO and survivin in well-to-moderately differentiated colorectal adenocarcinomas. On the basis of these results, we consider that the expression of Smac/DIABLO in colorectal carcinoma may be upregulated to suppress the anti-apoptotic effect of survivin.

最初，我们研究了磷酸化的caspase 9是否是有丝分裂灾难的新标记物。然而，这一点很难证明。接下来，我们用免疫组织化学方法检测Survivin和Smac/DIABLO在胃癌和结直肠癌中的表达。我们观察到，在胃癌和结直肠癌中，Survivin和Smac/Diablo的表达水平不同；结直肠癌中这两种表达均显著高于胃癌。此外，我们还发现Smac/DIABLO和Survivin在高到中等分化的结直肠腺癌中的表达之间存在相关性。基于这些结果，我们认为Smac/DIABLO在结直肠癌中的表达可能上调，从而抑制Survivin的抗凋亡作用。

项目成果

Immunohistochemical expression of nuclear and cytoplasmic survivin in gastrointestinal carcinoma.

• 发表时间: 2013-11
• 期刊: International journal of clinical and experimental pathology
• 影响因子: 1.4
• 作者: M. Shintani;Akiko Sangawa;N. Yamao;S. Kamoshida

胃癌および大腸癌におけるsurvivinの細胞内局在に関する検討

胃癌和结直肠癌中survivin的细胞内定位研究

• 发表时间: 2013
• 作者: Takeuchi M;Sato Y;Yasui H;Ozawa H;Ohno K;Takata K;Gion Y;Orita Y;Tachibana T;Itoh T;Asano N;Nakamura S;Swerdlow SH;Yoshino T.;新谷路子
• 通讯作者: 新谷路子

Phosphorylation status of Akt and caspase-9 in gastric and colorectal carcinomas.

• 发表时间: 2014-05
• 期刊: International journal of clinical and experimental pathology
• 影响因子: 1.4
• 作者: Akiko Sangawa;M. Shintani;N. Yamao;S. Kamoshida

Cell Survival or Death? Chaputer 10「Role of Autophagy in Cancer」

细胞生存还是死亡？第10章“自噬在癌症中的作用”

• 发表时间: 2013
• 作者: Toshiaki Nakano;Tohru Mizumasa;Hisako Yoshida;Masahiro Eriguchi;Masatomo Taniguchi;Kosuke Masutani;Hideki Hirakata;Kazuhiko Tsuruya;Takanari Kitazono;Michiko Shintani et al
• 通讯作者: Michiko Shintani et al

Autophagy - A Double-Edged Sword - Cell Survival or Death?

自噬——一把双刃剑——细胞生存还是死亡？

• 发表时间: 2013
• 作者: Shigeoka M;Urakawa N;Nakamura T;Nishio M;Watajima T;Kuroda D;Komori T;Kakeji Y;Semba S;Yokozaki H;Michiko Shintani
• 通讯作者: Michiko Shintani