A study to explore the mechanism of hepatic metastasis of colon cancer through Heregulin

赫调节蛋白探讨结肠癌肝转移机制的研究

• 批准号: 23590423
• 负责人: YOSHIOKA Toshiaki
• 金额: $ 3.24万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590423/
• 关键词: 肝転移; 大腸癌; Heregulin; 肝細胞; 大腸癌肝転移; 肝細胞傷害; 分子標的療法; 肺転移

We investigated a mechanism why hepatocytes express Heregulin(HRG) in the situation of hepatic metastasis of colorectal cancer. Immunohistochemical(IHC) examination on human specimen of hepatic metastasis revealed that HRG was expressed in hepatocytes at 88.7 %. The expression was evident in hepatocytes which were close to the metastatic tumor but not in those which were contact to the tumor with being injured nor in far region. Dimethylnitrosoamine(DMN) caused significantly increased expression of HRG mRNA in rat hepatocytes from day 1 compared with that of the control. IHC examination revealed that HRG expression in hepatocytes started from day 1 around the area of Glisson's capsule but not in the area of central zone of hepatic lobules where were injured by DMN. Partial hepatectomy also revealed that hepatocytes in the residual liver expressed HRG. These results suggest that HRG expression is appeared in the residual hepatocytes in the stage of injury and regeneration of the liver.

我们研究了大肠癌肝转移时肝细胞表达调蛋白（HRG）的机制。免疫组织化学（IHC）检测肝转移癌标本显示HRG在肝细胞中的表达率为88.7%。在肿瘤周围肝细胞中表达明显，而在肿瘤周围及远处肝细胞中表达不明显。与对照组相比，二甲基亚硝胺（DMN）从第1天起引起大鼠肝细胞HRG mRNA表达显著增加。免疫组化结果显示，HRG在肝细胞中的表达始于Glisson囊周围，而在DMN损伤的肝小叶中央带区域则无表达。部分肝切除术也显示，肝细胞在残留的肝脏表达HRG。提示HRG在肝损伤和再生阶段的残留肝细胞中有表达。

项目成果

Study of expression of heregulin in the human liver metastasis

调蛋白在人肝转移瘤中表达的研究

• 发表时间: 2012
• 作者: Yoshioka;T.;Nishikawa;Y.;Yamamoto;Y.;Omori;Y.;Nanjo;H.;Enomoto;K.
• 通讯作者: K.

Isthmin targets cell-surface GRP78 and triggers apoptosis via induction of mitochondrial dysfunction

• DOI: 10.1038/cdd.2014.3
• 发表时间: 2014-05-01
• 期刊: CELL DEATH AND DIFFERENTIATION
• 影响因子: 12.4
• 作者: Chen, M.;Zhang, Y.;Ge, R.
• 通讯作者: Ge, R.

新規抗HER3ラットモノクローナル抗体のヒト癌に対する反応性と抗腫瘍効果

新型抗 HER3 大鼠单克隆抗体对人类癌症的反应性和抗肿瘤作用

• 发表时间: 2013
• 作者: 沖田 鋼季;上田 詩歩;常盤 遼;八木 秀樹;井上 正宏;吉岡 年明;益子 高
• 通讯作者: 益子 高

肝転移巣周辺部および傷害肝における肝細胞でのheregulin発現の検討

肝转移和受损肝脏周围肝细胞中调蛋白表达的检查

• 发表时间: 2011
• 作者: 吉岡年明;西川祐司;山本洋平;大森泰文;南條博;榎本克彦
• 通讯作者: 榎本克彦

Cytoplasmic connex in 32 and self-renewal of cancer stem cells : Implication in metastasis. (2012) In "Hepatocellular Carcinoma – Basic Research" (ed) Lau W-Y, pp235-252

32 中的细胞质连接和癌症干细胞的自我更新：转移的意义（2012）见“肝细胞癌 - 基础研究”（ed）Lau W-Y，第 235-252 页。

• 发表时间: 2012
• 作者: Omori;Y.;Kawasaki;Y.;Li;Q.;Yoshioka;T.;Yamamoto;Y. and Enomoto;K.
• 通讯作者: K.