Identification of Pancreatic Cancer Stem Cells and Development of Novel Therapeutic Strategies using Stem Cell Markers

胰腺癌干细胞的鉴定和利用干细胞标记物开发新的治疗策略

• 批准号: 23590477
• 负责人: NAITO Zenya
• 金额: $ 3.16万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590477/
• 关键词: 癌幹細胞; 膵癌; 前癌病変; 癌幹細胞マーカー

The cancer stem cell markers for human pancreatic ductal adenocarcinoma are expressed in precancerous lesions, PanIN and IPMNs. The expression levels had increased strongly higher dysplasia in the precancerous lesions and marked overexpression was observed in adenocarcinomas. The CD133 overexpression in adenocarcinoma correlated with venous invasion. CD24, ESA and CXCR4 expressions in adenocarcinomas were related with well-differentiated types. The Ki-67 and MCM2 labeling indices of the cell proliferation markers was greater in higher grade precancerous lesions compered to low grade precancerous lesions, and increased markedly in carcinoma in situ and in invasive lesions. The newly ductal adenocarcinoma cell line that we established showed an overexpression of CXCR4. This cell line and 2 adenosquamous cell lines showed high levels of expression of ESA and MCM2. These cell lines were not tumorgenic in nude mice.

人胰腺导管腺癌的肿瘤干细胞标记物在癌前病变、Panin和IPMN中表达。在癌前病变中，高异型增生的表达水平明显升高，在腺癌中明显过表达。CD133在腺癌中的过度表达与静脉侵犯有关。CD24、ESA和CXCR4在腺癌中的表达与高分化类型有关。细胞增殖标记物的Ki-67和MCM2标记指数在高级别癌前病变和低度癌前病变中较大，在原位癌和浸润性病变中显著增加。我们建立的新的导管腺癌细胞系CXCR4过表达。该细胞株和2株腺鳞癌细胞株均高表达ESA和MCM2。这些细胞系在裸鼠体内不具有致瘤性。

项目成果

Ultra-high-resolution Images of Nestin and Vimentin in Pancreatic Carcinoma Cells Using 2 Novel Microscopy Systems

使用 2 种新型显微镜系统获得胰腺癌细胞中巢蛋白和波形蛋白的超高分辨率图像

• DOI: 10.1272/jnms.79.392
• 发表时间: 2012
• 期刊: Journal of Nippon Medical School
• 影响因子: 1
• 作者: Yoshimura H;Matsuda Y;Naito Z;Murase M;Kawamoto Y;Ishiwata T
• 通讯作者: Ishiwata T

Immunocytochemical analysis of a three-dimensional spheroidal culture system

三维球状培养系统的免疫细胞化学分析

• 发表时间: 2011
• 期刊: J Nippon Med Sch
• 影响因子: 1
• 作者: Matsuda Y;Ishiwata T;Naito Z
• 通讯作者: Naito Z

手術検体からの膵癌培養細胞樹立の検討

手术标本培养胰腺癌细胞的研究

• 发表时间: 2013
• 作者: 川原清子;松田陽子;中澤南堂;河本陽子;鈴木妙子;石渡俊行;内田英二;内藤善哉
• 通讯作者: 内藤善哉

Availability of cytological diagnoses in pancreatobiliary tract diseases and cytological slide seminar of pancreatobiliary tract lesions

胰胆道疾病细胞学诊断的可用性及胰胆道病变细胞学切片研讨会

• 发表时间: 2013
• 作者: Naito Z ;Peng W-X;Isobe H;Katayama H;Hosone M;Tanno M;Maeda S
• 通讯作者: Maeda S

NOGマウス膵癌転移モデルの転移細胞株における細胞動態の検討

NOG小鼠胰腺癌转移模型转移细胞系的细胞动力学检查

• 发表时间: 2011
• 作者: 川原清子;松田陽子;藤井雄文;山初和也;上田純志;石渡俊行;内藤善哉
• 通讯作者: 内藤善哉