Role of lumican for cell growth and differentiation in pancreatic cancer

Lumican 对胰腺癌细胞生长和分化的作用

• 批准号: 17591445
• 负责人: NAITO Zenya
• 金额: $ 1.92万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591445/
• 关键词: pancreatic cancer; pancreatitis; lumican; cell growth; proteoglycan; immunohistochemistry; 膵臓癌

Lumican is a member of a small leucine-rich proteoglycan family and its overexpression has been reported in breast, colorectal, uterine cervical and pancreatic cancers. The expression of lumican in stromal tissues in breast cancer is associated with a high tumor grade, a low estrogen receptor expression level, and young age. Lumican expression in the cytoplasm in advanced colorectal cancer correlated with poor prognosis. Lumican expression was previously reported in pancreatic cancer and adjacent stromal tissues. Reverse-transcription polymerase chain reaction (RT-PCR) and Western blot analyses revealed lumican mRNA and protein expression in six pancreatic ductal adenocarcinoma (PDAC) cell lines (PANC-1, MIA PaCa-2, KLM-1, Capan-1, PK-1 and PK-8). On the basis of its immunoreactivity, lumican was found to be localized in islet cells of normal pancreatic tissues. In pancreatic cancer tissues, lumican was localized in the cytoplasm of cancer cells in 30 of 53 (57%) cancer patients, whereas lumican was detected in stromal tissues in 36 of 53 (68%) cancer patients. Lumican expression in pancreatic cancer cells did not correlate with clinicopathological factors, whereas lumican expression in stromal tissues correlated with the female gender, advanced stage, retroperitoneal and duodenal invasion. Patients with lumican-positive stromal tissues survived shorter than those with lumican-negative stromal tissues. These results suggest that lumican in stromal tissues play important roles in the growth and invasion of pancreatic cancer. Different glycosylated side chains of lumican may present in cytoplasm of cancer cells and adjacent stromal tissues.

Lumican是一个富含亮氨酸的蛋白聚糖小家族的成员，其过表达已在乳腺癌、结直肠癌、宫颈癌和胰腺癌中报道。乳腺癌间质组织中Lumican的表达与高肿瘤分级、低雌激素受体表达水平和年轻相关。Lumican在进展期结直肠癌胞浆中的表达与预后不良相关。Lumican的表达以前曾在胰腺癌和邻近的间质组织中报道过。逆转录聚合酶链反应（RT-PCR）和蛋白质印迹分析揭示了6种胰腺导管腺癌（PDAC）细胞系（PANC-1、MIA PaCa-2、KLM-1、Capan-1、PK-1和PK-8）中lumican mRNA和蛋白质的表达。基于其免疫反应性，发现Lumican定位于正常胰腺组织的胰岛细胞中。在胰腺癌组织中，在53名癌症患者中的30名（57%）中，Lumican定位于癌细胞的细胞质中，而在53名癌症患者中的36名（68%）中，在基质组织中检测到Lumican。Lumican在胰腺癌细胞中的表达与临床病理因素无关，而Lumican在间质组织中的表达与女性性别、晚期、腹膜后和十二指肠浸润相关。具有Lumican阳性基质组织的患者存活时间短于具有Lumican阴性基质组织的患者。提示间质组织中的Lumican在胰腺癌的生长和侵袭中起重要作用。不同的糖基化的Lumican侧链可以存在于癌细胞和邻近的基质组织的细胞质中。

项目成果

Lumican expression in advanced colorectal cancer with nodal metastasis correlates with poor prognosis.

• DOI: 10.3892/or.16.6.1225
• 发表时间: 2006-12
• 期刊: Oncology reports
• 影响因子: 4.2
• 作者: T. Seya;N. Tanaka;S. Shinji;K. Yokoi;M. Koizumi;Nobuhisa Teranishi;K. Yamashita;T. Tajiri;T. Ishiwata;Z. Naito

Role of small leucine-rich proteoglycan (SLRP) family in pathological lesions and cancer cell growth

小富含亮氨酸蛋白聚糖（SLRP）家族在病理病变和癌细胞生长中的作用

• 发表时间: 2005
• 期刊: J Nippon Medical School 72・3
• 作者: Kuriu Y;Otsuji E;et.;Tomoko Seya;Tomoko Seya;Zenya Naito
• 通讯作者: Zenya Naito

Role of lumican in cancer cells and adjacent stromal tissues in human pancreatic cancer.

• DOI: 10.3892/or.18.3.537
• 发表时间: 2007-09
• 期刊: Oncology reports
• 影响因子: 4.2
• 作者: T. Ishiwata;Kazumitsu Cho;K. Kawahara;Tetsushi Yamamoto;Yuri Fujiwara;E. Uchida;T. Tajiri;Z. Naito