Preclinical study to elucidate molecular mechanism of matrix anchoring using bacterial proteins

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Flesh-eating bacteria produce collagenases, enzymes to degrade tissue collagen fibrils. The enzyme possesses a collagen-binding domain(s). Although its molecular size is small, the domain forms sandwich-like structure to bind to untwisted region of triple-helical collagen molecule. Meanwhile, human growth factor accelerates cell growth. Various growth factors were fused with the domain to anchor them on tissue collagen fibrils, demineralized bone matrix, or collagen sheet/particles. Using these novel materials, osteogenesis or angiogenesis was successfully induced.