Study on structural biology of novel fibronectin-binding proteins of a pathogenic bacterium

一种病原菌新型纤连蛋白结合蛋白的结构生物学研究

• 批准号: 23590524
• 负责人: KATAYAMA Seiichi
• 金额: $ 3.41万
• 依托单位: Okayama University of Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590524/
• 关键词: Clostridium perfringens; fibronectin (Fn); Fn-binding protein; Fn III1-C-binding site; ウェルシュ菌; ガス壊疽; フィブロネクチン (Fn); Fn結合タンパク質; 国際情報交流; フィブロネクチン（Fn)

Clostridium perfringens is a pathogenic bacterium, causing gas gangrene and food poisoning in humans and animals. During the beginning of the infection, the bacteria formed colonies after the adhesion to wounds and epithelial cells of intestine. C. perfringens have proteins, FbpA (25 kDa, 220 aa) and FbpB (66 kDa, 575 aa) that bind to fibronectin in human. Therefore, this bacterium is thought to colonize after its Fn-binding.In this study, we indicated that Fn molecules bound to the C-terminal region of FbpA. An improved method of purification of recombinant FbpA was established to get a great quantity of the protein for crystallization. In addition, we found that both FbpA and FbpB could bind to the surface of C. perfringens cell. The studies on the structures and functions of the Fbps might lead to the development of protection against C. perfringens infection in the future.

产气荚膜梭菌是一种致病菌，可引起人畜气性坏疽和食物中毒。在感染初期，细菌粘附于伤口和肠上皮细胞后形成菌落。C.产气荚膜菌具有与人纤维连接蛋白结合的蛋白FbpA （25 kDa, 220 aa）和FbpB （66 kDa, 575 aa）。因此，这种细菌被认为是在与fn结合后定植的。在这项研究中，我们发现Fn分子结合在FbpA的c端区域。建立了一种改进的纯化重组FbpA的方法，以获得大量的蛋白用于结晶。此外，我们发现FbpA和FbpB都能与产气荚膜梭菌细胞表面结合。对Fbps的结构和功能的研究为今后开发抗产气荚膜荚膜原菌感染的防护技术奠定了基础。

项目成果

ウェルシュ菌フィブロネクチン(Fn)結合タンパク質FbpAのFn III_1-C結合ドメインの探索

搜索产气荚膜梭菌纤连蛋白 (Fn) 结合蛋白 FbpA 的 Fn III_1-C 结合域

• 发表时间: 2013
• 作者: 岡田麻里;櫃本泰雄;片山誠一
• 通讯作者: 片山誠一

Fibronectin (Fn)-binding proteins of Clostridium perfringens recognizes III1-C-expressing serum Fn

产气荚膜梭菌的纤连蛋白 (Fn) 结合蛋白识别表达 III1-C 的血清 Fn

• 发表时间: 2013
• 作者: Tsutom Yamasaki;Yoko Seike;Hatsuo Yamashita;Seiichi Katayama;Yasuo Hitsumoto
• 通讯作者: Yasuo Hitsumoto

Clostridium perfringens由来フィブロネクチン結合タンパクFbpAの好中球遊走に与える影響

产气荚膜梭菌来源的纤连蛋白结合蛋白 FbpA 对中性粒细胞迁移的影响

• 发表时间: 2013
• 作者: 山下初津雄;山崎勤;片山誠一;櫃本泰雄
• 通讯作者: 櫃本泰雄

Adhesive properties of Clostridium perfringens to extracellular matrix proteins collagens and fibronectin

• DOI: 10.1016/j.anaerobe.2013.11.002
• 发表时间: 2014-02-01
• 期刊: ANAEROBE
• 影响因子: 2.3
• 作者: Hitsumoto, Yasuo;Morita, Naomi;Katayama, Seiichi
• 通讯作者: Katayama, Seiichi

Determination of binding site for Clostridium perfringens on fibronectin

产气荚膜梭菌在纤连蛋白上结合位点的测定

• 发表时间: 2013
• 作者: Seiichi Katayama;Mika Tagomori;Hatsuo Yamashita;Tsutomu Yamasaki;Hirofumi Nariya;Mariko Okada;Mariko Watanabe;Yasuo Hitsumoto
• 通讯作者: Yasuo Hitsumoto