Analysis of Restriction Factors of cynomolgus monkey in establishment of HIV-1/monkey model

食蟹猴HIV-1/猴模型建立的限制因素分析

• 批准号: 23590541
• 负责人: NAKAYAMA Emi
• 金额: $ 3.41万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590541/
• 关键词: ヒト免疫不全ウイルス; 感染抑制因子; 動物モデル; HIV-1感染; TRIM5α; TRIMCyp; TRIM5; cynomolgus monkey; HIV-1

TRIM5alpha was previously identified as an intrinsic factor that restricts HIV-1 replication in rhesus monkey cells. Thereafter, TRIMCyp was discovered as a mutated allele of TRIM5 gene. We demonstrated that the frequencies of TRIMCyp in cynomolgus monkey markedly higher than that of rhesus monkey. Then, we infected monkey-tropic HIV-1 (MN4Rh-3) to TRIMCyp homozygotes or TRIM5alpha homozygotes. The viral growth was higher in TRIMCyp homozygotes than that in TRIM5alpha homozygotes. These results indicated that genotyping of TRIM5 gene is important to select monkey susceptible to monkey-tropic HIV-1 in establishment of HIV-1/monkey model system.

TRIM 5 α先前被鉴定为限制HIV-1在恒河猴细胞中复制的内在因子。此后，TRIMCyp被发现为TRIM 5基因的突变等位基因。结果表明，食蟹猴TRIMCyp基因频率明显高于恒河猴。然后，我们将嗜猴HIV-1（MN 4 Rh-3）感染至TRIMCyp纯合子或TRIM 5 α纯合子。TRIMCyp纯合子的病毒生长量高于TRIM 5 α纯合子。这些结果表明，在建立HIV-1/猴模型系统时，TRIM 5基因分型对筛选猴嗜性HIV-1易感猴具有重要意义。

项目成果

Diversity of antiretroviral host factor TRIM5 gene in macaque monkeys

猕猴抗逆转录病毒宿主因子TRIM5基因的多样性

• 发表时间: 2013
• 作者: 金澤伯弘;川畑淑子;永野希織;小原道法;小澤真;小原恭子.;Akatsuki Saito
• 通讯作者: Akatsuki Saito

Slower uncoating has a deleterious effect on replication of the simian-tropic HIV-1.

较慢的脱壳对猿类 HIV-1 的复制具有有害影响。

• 发表时间: 2013
• 作者: Ken Kono;Eri Takeda;Hiromi Tsutui;Ayumu Kuroishi;Amy E. Hulme;Thomas J. Hope;Emi E. Nakayama;Tatsuo Shioda.
• 通讯作者: Tatsuo Shioda.

Genetic Diversity of TRIM5 Gene and HIV-1 Susceptibility in Cynomolgus Magaque(Macaca Fascicularis)

食蟹猴TRIM5基因遗传多样性与HIV-1易感性

• 发表时间: 2012
• 作者: 前田和彦、Almofty SA;Singh SK;Eid MMA,下田真唯子,池田輝政、小糸厚、坂口薫雄;Emi E. Nakayama
• 通讯作者: Emi E. Nakayama

A naturally occurring single amino acid substitution in human TRIM5α linker region affects its anti-HIV-1 activity and susceptibility to HIV-1 infection

人 TRIM5α 连接区中天然存在的单个氨基酸取代会影响其抗 HIV-1 活性和对 HIV-1 感染的易感性

• DOI: 10.1089/aid.2012.0369
• 发表时间: 2013
• 期刊: AIDS Res Hum Retroviruses
• 作者: Nakayama EE;Nakajima T;Kaur G;Mimaya JI;Terunuma H;Mehra N;Kimura A;Shioda T
• 通讯作者: Shioda T

Systemic biological analysis of the mutations in two distinct HIV-1mt genomes occurred during replication in macaque cells

• DOI: 10.1016/j.micinf.2013.01.005
• 发表时间: 2013-04-01
• 期刊: MICROBES AND INFECTION
• 影响因子: 5.8
• 作者: Nomaguchi, Masako;Doi, Naoya;Adachi, Akio
• 通讯作者: Adachi, Akio