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New method of cat scratch disease by ELISA using specific antigens and its application to the pathological clarification

New method of cat scratch disease by ELISA using specific antigens and its application to the pathological clarification
特异性抗原ELISA检测猫抓病新方法及其在病理澄清中的应用
批准号:
23590673
负责人:
TSUNEOKA Hidehiro
金额:
$3.33万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2011
资助国家:
日本
项目状态:
已结题
起止时间:
2011 至 2013

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中文摘要
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英文摘要
The measurement of Bartonella henselae IgG by highly-sensitive ELISA requires highly-specific purification of antigen. The extract of B. henselae ATCC49882 by N-lauroyl sarcosinate includes a great deal of useful protein component. We directly extracted B. henselae ATCC49882 by N-lauroyl sarcosinate for 2-4 times without supersonic treatment and purified it by diethlaminoethyl ion-exchange (DEAE) chromatography. As a result, it was clarified that highly-specific protein components were eluted with the salt concentration of 245-295mM. Further development of ELISA and its availability by using this antigenic component will be greatly expected.
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MORPHOLOGICAL STUDY OF ENCEPHALOPATHY CAUSED BY INFECTION WITH BARTONELLA HENSELAE
汉塞巴尔通体感染引起的脑病的形态学研究
DOI: --
发表时间: 2011
期刊:
影响因子: --
作者: [岡田侑也, 重藤翔平, 奥宮敏可, Akiko Umeda]
通讯作者: Akiko Umeda
パスツレラによる肺炎の1例
巴氏杆菌引起的肺炎一例
DOI: --
发表时间: 2012
期刊:
影响因子: --
作者: [山中万次郎, 山川智弘, 奥宮敏可, 砂川将直]
通讯作者: 砂川将直
Real-time PCR法によるBartonella henselae感染症の迅速診断法の開発とその有用性
实时荧光定量PCR法快速诊断汉赛巴尔通体感染方法的建立及其实用性
DOI: --
发表时间: 2012
期刊:
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作者: [井手俊明, 岡田侑也, 奥宮敏可, 柳原正志]
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Prosthetic valve endocarditis caused by Bartonella quintana in a patient during immunosuppressive therapies for collagen vascular diseases
胶原血管疾病免疫抑制治疗期间患者五体巴尔通体引起的人工瓣膜心内膜炎
DOI: 10.1016/j.diagmicrobio.2011.03.011
发表时间: 2011
期刊: Diagnostic Microbiology and Infectious Disease
影响因子: 2.9
作者: [Yuki Yamada, Kiyofumi Ohkusu, Masashi Yanagihara, Hidehiro Tsuneoka]
通讯作者: Hidehiro Tsuneoka
21
    Establishment of highly sensitive ELISA to determine specific IgM antibody for diagnosis of acute cat scratch disease and elucidation of its pathology
    • 批准号:
      17K09013
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $3.08万
    • 财政年份:
      2017
    • 负责人:
      TSUNEOKA Hidehiro
    • 依托单位:
    Detection and pathological analyses of specific antibody in cat scratch disease by highly-sensitive ELISA using Japanese-derived strain
    • 批准号:
      26460678
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $3.24万
    • 财政年份:
      2014
    • 负责人:
      TSUNEOKA Hidehiro
    • 依托单位:
    Improved method of ELISA for cat scratch disease
    • 批准号:
      20590574
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $3.0万
    • 财政年份:
      2008
    • 负责人:
      TSUNEOKA Hidehiro
    • 依托单位:
    海外基金