Searching for neurodegenerative disease-related factors in the metabolic systems of ADMA-containing protein and the elucidation of the functional mechanism

为了证明中枢神经系统不对称二甲基精氨酸（ADMA）代谢途径的生理意义，我们检测了ADMA代谢酶PRMT1、DDAH1和nNOS在大鼠脊髓和其他脑区的免疫组织化学定位。Western blot分析显示PRMT1和DDAH1分布广泛。免疫组织化学分析表明，这些酶在许多神经元中都有共定位。在皮层和下丘脑，几乎所有的nNOS神经元都有PRMT1和DDAH1。此外，我们通过蛋白质组学分析调查了大鼠脑中未知的精氨酸甲基化蛋白，并鉴定出果糖1,6-二磷酸醛缩酶C （FBAC）是一种新的含adma的蛋白。提示PRMT/DDAH/NOS轴在中枢神经系统中发挥稳态功能。

To demonstrate the physiological significance of the metabolic pathways for asymmetric dimethylarginine (ADMA) in central nervous system, we examined the immunohistochemical localization of ADMA metabolizing enzymes, PRMT1 and DDAH1 and nNOS in the rat spinal cord and the other brain regions. Western blot analyses showed that PRMT1 and DDAH1 were widely distributed. The immunohistochemical analyses showed that these enzymes colocalized in a lot of neurons. In the cortex and hypothalamus, almost all nNOS neurons had PRMT1 and DDAH1. Additionally, we surveyed unknown arginine methylated proteins from rat brain by proteomic analysis and identified fructose 1,6-bisphosphate aldolase C (FBAC) as a novel ADMA-containing protein. These findings suggest that the PRMT/DDAH/NOS axis is play a part of homeostatic function in central nervous system.