Role of GPR103-26RFa-QRFP system in the development of neuropathic pain

GPR103-26RFa-QRFP系统在神经病理性疼痛发生中的作用

• 批准号: 23590715
• 负责人: TANAHIRA Chiyoko
• 金额: $ 3.08万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590715/
• 关键词: GPR108; orphan receptor; 神経障害性疼痛; 炎症性疼痛; 可塑性; 神経科学; 神経伝達物質; 痛み; GPR103; 26RFa; QRFP; ノックアウトマウス

In the present study, the effects of intrathecal and intracerebroventricular application of 26RFa, on the level of mechanical allodynia induced by partial sciatic nerve ligation, were examined in rats. Intrathecal and intracerebroventricular injection of 26RFa attenuated the level of mechanical allodynia in a dose dependent manner. Immunohistochemical examination revealed that QRFP-like immunoreactivity (QRFP-LI) was expressed mainly in the small to medium sized neuronal profiles in the L5 dorsal root ganglion and that partial sciatic nerve injury increased the percentage of QRFP-LI positive neuronal profiles and made the cross-section area of the QRFP-LI positive neuronal profiles larger in the L5 dorsal root ganglion ipsilateral to the nerve injury.Microinjection of 5 g of 26RFa into RVM decreased the number of flinching behavior induced by paw formalin injection significantly as compared with saline injected rats. This effect of 26RFa was not antagonized by naloxone.

在本研究中，在大鼠中检查了鞘内和侧脑室内应用26 RFa对部分坐骨神经结扎引起的机械性异常性疼痛水平的影响。鞘内和侧脑室注射26 RFa以剂量依赖的方式减轻机械性异常性疼痛的水平。 免疫组化结果显示，在L5背根神经节中，QRFP-LI主要表达于中小神经元，坐骨神经部分损伤可使损伤侧L5背根神经节中QRFP-LI阳性神经元比例增加，截面积增大，RVM内微量注射26 RFa 5g可显著减少福尔马林注射引起的大鼠缩足次数。纳洛酮不能拮抗26 RFa的这种作用。

项目成果

Microinjection of 26RFa, an endogeniou ligand for GPR103, into rostral ventromedial medulla (RVM) produces an analgesic effect in the rat formalin test

将 26RFa（GPR103 的内源性配体）显微注射到头端腹内侧延髓 (RVM)，在大鼠福尔马林试验中产生镇痛作用

• 发表时间: 2014
• 作者: Miki Araki;Takahiro Nonaka;Toshihiko Yamada;Tatsuo Yamamoto
• 通讯作者: Tatsuo Yamamoto

Anti-allodynic effects of intrathecally and intracerebroventricularly administered 26RFa, an intrinsic agonist for GRP103, in the rat partial sciatic nerve ligation model

鞘内和脑室内注射 26RFa（GRP103 的内在激动剂）在大鼠部分坐骨神经结扎模型中的抗异常疼痛作用

• 发表时间: 2011
• 期刊: Peptides
• 影响因子: 3
• 作者: Tatsuo Yamamoto;R. Miyazaki;Toshihiko Yamada;T. Shinozaki
• 通讯作者: T. Shinozaki

Microinjection of Neuropeptide W, an endogeniou ligand for GPR7 and 8, into rostral ventromedial medulla (RVM) produces an analgesic effect in the rat formalin test

将神经肽 W（GPR7 和 8 的内源性配体）显微注射到延髓头端腹内侧 (RVM)，在大鼠福尔马林试验中产生镇痛作用

• 发表时间: 2014
• 作者: Takahiro Nonaka;Toshihiko Yamada;Miki Araki;Tatsuo Yamamoto
• 通讯作者: Tatsuo Yamamoto

Microinjection of 26RFa, an endogenous ligand for GPR103, into rostralventromedial medulla (RVM) produces an analgesic effect in the rat formalin test

将 26RFa（GPR103 的内源性配体）显微注射到延髓头腹内侧 (RVM)，在大鼠福尔马林试验中产生镇痛作用

• 发表时间: 2014
• 作者: Yasukochi Y;Takenaka K;Yurino A;Kurihara Y;Miake S;Uchi H;Takeuchi S;Kohda F;Moroi Y;Okano S;Yamamoto H;Furue M;Tatsuo Yamamoto
• 通讯作者: Tatsuo Yamamoto

Microinjection of Neuropeptide W, an endogenous ligand for GPR7 and 8, into rostral ventromedial medulla (RVM) produces an analgesic effect in the rat formalin test.

将神经肽 W（GPR7 和 8 的内源性配体）显微注射到延髓头端腹内侧 (RVM)，在大鼠福尔马林试验中产生镇痛作用。

• 发表时间: 2014
• 作者: 山田 宗治;尾田 高志;小松 秀平;藤井 理恵;大島 泰斗;廣瀬 剛;小島 糾;須藤 泰代;冨安 朋宏;吉川 憲子;吉田 雅治;Tatsuo Yamamoto
• 通讯作者: Tatsuo Yamamoto