Establishment of quick detection system of chikungunya-infected mosquitoes for preventing from an outbreak of the secondary-infected patients

建立基孔肯雅热蚊类快速检测系统，预防继发感染暴发

• 批准号: 23590803
• 负责人: ESHITA Yuki
• 金额: $ 3.08万
• 依托单位: Oita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590803/
• 关键词: 媒介蚊対策; 迅速遺伝子診断; チクングニア熱; 蚊; RT-LAMP; チクングニアウイルス; 感受性; リスク評価; 媒介蚊; ウイルスゲノム; 遺伝子検出

The susceptibility and risk evaluation of Japanese mosquitoes to chikungunya virus, and introduction of RT-LAMP method were evaluated in experimental conditions. It was revealed that the three mosquito species, Aedes albopictus, Ae. flavopictus, and Aedes riversi had transmission ability for the virus. In addition, the RT-LAMP method was superior to the RT-PCR method as the quick pathogen detection system. Aedes albopictus was estimated as the mosquito, which was the highest in a risk of the pathogen spread in Japan. Pathogen detection system including these results may contribute to prevent from an outbreak of the secondary-infected patients.

在实验条件下，对日本蚊对基孔肯雅病毒的敏感性和风险评价以及RT-LAMP方法的引入进行了评价。据透露,三个蚊子,白纹伊蚊,Ae。黄纹伊蚊、河伊蚊对该病毒具有传播能力。RT-LAMP法作为快速病原菌检测系统优于RT-PCR法。据估计，白纹伊蚊在日本的传播风险最高。包括这些结果的病原体检测系统可能有助于预防继发性感染患者的爆发。

项目成果

Polysaccharide Based Graft Copolymers

• DOI: 10.1007/978-3-642-36566-9
• 发表时间: 2013
• 期刊: Polysaccharide Based Graft Copolymers
• 作者: S. Kalia;M. Sabaa

Whole transcriptome analysis comparison of Aedes aegypti 6- and 14-day post-dengue infection using RNA-seq

使用 RNA-seq 对登革热感染后 6 天和 14 天的埃及伊蚊进行全转录组分析比较

• 作者: Lucky R. Runtuwene;Shuichi Kawashima;Yutaka Suzuki;Sumio Sugano;Kenta Nakai;Ryuichiro Maeda;Chihiro Sugimoto;Tomohiko Takasaki;Ichiro Kurane and Yuki Eshita
• 通讯作者: Ichiro Kurane and Yuki Eshita

病原性の単細胞生物とそれを媒介するベクター (ランチョンセミナー)

致病性单细胞生物及其载体（午餐研讨会）

• 作者: Hiyoshi M;Uemura H;Arisawa K;Nakamoto M;Hishida A;Okada R;Matsuo K;Kita Y;Niimura H;Kuriyama N;Nanri H;Ohnaka K;Suzuki S;Mikami H;Kubo M;Tanaka H;Hamajima N; J-MICC Study Group;江下優樹
• 通讯作者: 江下優樹

Whole transcriptome analysis of Aedes aegypti 14 days post-dengue infection using RNA-seq

使用 RNA-seq 对登革热感染后 14 天的埃及伊蚊进行全转录组分析

• 作者: Lucky R. Runtuwene;Shuichi Kawashima;Yutaka Suzuki;Sumio Sugano;Kenta Nakai;Ryuichiro Maeda;Chihiro Sugimoto;Tomohiko Takasaki;Ichiro Kurane and Yuki Eshita
• 通讯作者: Ichiro Kurane and Yuki Eshita

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