Establishment of quick detection system of chikungunya-infected mosquitoes for preventing from an outbreak of the secondary-infected patients

在实验条件下评价了日本蚊虫对基孔肯雅病毒的敏感性和风险评估，以及RT-LAMP方法的引入。结果表明，白纹伊蚊Aedes albopictus，Ae.黄纹伊蚊和河伊蚊对病毒具有传播能力。此外，RT-LAMP方法作为快速病原菌检测系统优于RT-PCR方法。据估计，白纹伊蚊是这种病原体在日本传播风险最高的蚊子。病原体检测系统包括这些结果可能有助于防止二次感染患者的暴发。

The susceptibility and risk evaluation of Japanese mosquitoes to chikungunya virus, and introduction of RT-LAMP method were evaluated in experimental conditions. It was revealed that the three mosquito species, Aedes albopictus, Ae. flavopictus, and Aedes riversi had transmission ability for the virus. In addition, the RT-LAMP method was superior to the RT-PCR method as the quick pathogen detection system. Aedes albopictus was estimated as the mosquito, which was the highest in a risk of the pathogen spread in Japan. Pathogen detection system including these results may contribute to prevent from an outbreak of the secondary-infected patients.