Pathophysiological correlation between fibrogenesis and regeneration in murine liver via Notch/Jagged-1 signal

为了阐明Notch信号在肝脏纤维发生和再生之间的相关性，我们通过蛋氨酸/胆碱缺乏饮食（MCD）肝纤维化模型检测了Notch信号的表达。小鼠在MCD饮食开始后的第2、4和8周被处死。免疫印迹法观察Notch受体和Notch配体在肝脏中的表达。MCD组与对照组在肝纤维化过程中Notch信号的表达差异无统计学意义。在我们使用条件Mx/Cre Jagged-1敲除小鼠的初步数据中，我们推测Notch信号调节了肝纤维化中新生和再生的相关性。然而，在MCD模型中，Notch信号不参与肝纤维化。通过使用其他肝纤维化模型，阐明Notch信号在肝脏纤维化过程中的作用还需要进一步的研究。

To clarify the involvement of Notch signal in the correlation between fibrogenesis and regeneration in the liver, expression of Notch signal was examined by methionine/choline-deficient diet (MCD) model of liver fibrosis. Mice were sacrificed at 2, 4, and 8 weeks after MCD diet started. Hepatic expression of Notch receptors and Notch ligands were investigated by immunoblot. There were no significant differences between MCD group and control group in the expression of Notch signal during the progression of liver fibrosis. In our preliminary data by using conditional Mx/Cre Jagged-1 knockout mice, it was speculated that Notch signal modulates the correlation between fbrogenesis and regeneration in liver fibrosis. However, in MCD model, Notch signal was not involved in hepatic fibogrnesis. By using other liver fibrosis models, further studies are necessary for the elucidation of the role of Notch signal in the fibrogenic process in the liver.