Development of transplantation of hepatocytes produced from iPS cells to liver failure

点击翻译按钮获取中文摘要

A medium with Activin A was developed for feeder-free culture of iPS cells. iPS cells differentiated to hepatoblasts from iPS cells transfected with C/EBP alpha, GATA4, HEX, and FoxA2 in a medium with Oncostatin M, EGF, and RA. Hepatocyte selection medium was developed without glucose or arginine, supplemented with galactose and ornithine. When HSM was added to co-culture of iPS cells and human primary hepatocytes, iPS cells died and hepatocytes survived. Hepatocytes were successfully purified.