Cyclopedic investigation of genes for NASH-mediated carcinogenesis

我们使用原生cgh微阵列分析检测了胆碱缺乏l -氨基酸定义（CDAA）饮食诱导的nash相关肝癌的基因表达。48周的CDAA饮食诱导了肝脏中52个基因的表达差异。其中包括肿瘤增殖相关基因，以及转录调节基因HNF1/TCF1的表达按时间顺序减弱。NF1已知与信号事务相关，按时间顺序递减。Real - time PCR结果显示，CDAA饲料有降低肝脏中HNF1/TCF1的趋势，而肝脏中NF1 mRNA的表达没有变化。这些结果产生了同源cgh微阵列分析和实时PCR之间的基因表达差异。未来还需要进一步的调查。

We examined the genes expressions for NASH-related hepatocarcinogenesis induced by choline-deficient L-amino acid-defined (CDAA) diet, using origo-CGH microarray analysis. Forty-eight week CDAA diet iuduced 52 genes expression differences in the liver between CDAA diets and control. These included tumor proliferation-related genes, and HNF1/TCF1, transcriptional regulation genes expressions were chronologically attenuated. NF1 which is known to be related signal transaction, are chronologically decreased. Real time PCR showed that HNF1/TCF1 has a tendency to be decreased by CDAA diet, however, NF1 mRNA expressions in the liver were not changed by CDAA diet. These results produced the gene expression differences between origo-CGH microarray analysis and real time PCR. Further investigations were needed to examine in the future.