A study for the role of central neuropeptides on severe acute pancreatitis

本实验观察了中枢促甲状腺激素释放激素(TRH)对大鼠胰腺血流量和实验性急性胰腺炎的影响。1)脑池内注射TRH类似物(30 Ng)后，30min胰腺血流量增加，90min后恢复到基线水平；(2)TRH类似物对胰腺血流量的刺激作用在3~10 ng之间呈剂量依赖关系。(3)脑池内TRH类似物对胰腺血流量的刺激作用可被阿托品、L和迷走神经切断阻断。4)脑池内注射TRH类似物(30-50 ng)可剂量依赖性地抑制雨蛋白注射所致的胰腺肿胀。5)微量注射方法显示迷走神经背侧运动核是TRH刺激胰腺血流的反应部位。

Effects of central thyrotropin-releasing hormone (TRH) on pancreatic blood flow and experimental acute pancreatitis were investigated in male Wistar rats.1)Intracisternal injection of TRH analog (30 ng) induced an increase in the pancreatic blood flow at 30 minutes and the stimulated blood flow was returned to the basal line at 90 minutes.2)The stimulatory effect of intracisternal TRH analog on the pancreatic blood flow was dose-related ranging from 3 to 10 ng.3)The stimulatory effect of intracisternal TRH analog on the pancreatic blood flow was blocked by atropine, L-NAME and vagotomy, but not by 6-hydroxydopamine.4)Intracisternal injection of TRH analog (30- 50 ng) dose-dependently inhibited the pancreatic edema induced by cerulein injection.5)Microinjection methods revealed the dorsal vagal motor nucleus is responsive site for TRH to induce stimulation of the pancreatic blood flow.