Research for the mechanism of aldosterone synthesis in the adrenal gland and the heart.

研究肾上腺和心脏醛固酮合成机制。

• 批准号: 23591089
• 负责人: YOSHIMURA Michihiro
• 金额: $ 3万
• 依托单位: Jikei University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591089/
• 关键词: アルドステロン; 心筋細胞; 高血糖; 虚血再灌流障害; 鉱質コルチコイド受容体; p38 MAPK; 循環器; 病態生理; 生理活性物質

We aimed at the establishment of the experimental system which showed small quantity of aldosterone (Aldo) synthesized in the heart. Various conditions of culture medium and time course were examined using the cultured neonatal rat cardiomyocytes (NRCs). We found that CYP11B2 gene was expressed by short time stimulation of high glucose. This system will be extensively utilizable in the future research of cardiac Aldo synthesis. On the other hand, an unknown action of Aldo was examined by using the Langendorff system. It was found that the pre-treatment of Aldo attenuated ischemia reperfusion injury, suggesting that Aldo per se has a preconditioning action. Also, we newly established the cell culture system which showed the precise intracellular sodium concentration. By this system, we could measure the absolute increase in the intra-cellular sodium concentration stimulated by Aldo and cortisol. This system is also utilizable for the future studies of cardiac hypertrophy and others.

目的：建立心脏合成少量醛固酮的实验体系。使用培养的新生大鼠心肌细胞（NRC）检查各种培养基条件和时间过程。我们发现，CYP11B2基因在高糖短时间刺激下表达。该系统在心脏醛合成的研究中具有广阔的应用前景。另一方面，通过使用Langendorff系统，研究了Aldo的未知作用。结果表明，Aldo预处理可减轻缺血再灌注损伤，提示Aldo本身具有预处理作用。此外，我们新建立的细胞培养系统，显示了精确的细胞内钠浓度。通过该系统，我们可以测量由Aldo和皮质醇刺激的细胞内钠浓度的绝对增加。该系统也可用于未来的研究心脏肥大和其他。

项目成果

Aldosterone Preconditioning is Protective during Ischemia-Reperfusion through Modulation of p38 MAPK Signaling in Isolated Rat Heart

醛固酮预处理通过调节 p38 MAPK 信号在离体大鼠心脏缺血再灌注过程中发挥保护作用

• 发表时间: 2014
• 作者: 吉野拓哉;吉村道博;他
• 通讯作者: 他

A technique for quantifying intracellular free sodium ion using a microplate reader in combination with sodium-binding benzofuran isophthalate and probenecid in cultured neonatal rat cardiomyocytes.

• DOI: 10.1186/1756-0500-6-556
• 发表时间: 2013-12-26
• 期刊: BMC research notes
• 影响因子: 1.8
• 作者: Katoh D;Hongo K;Ito K;Yoshino T;Kayama Y;Komukai K;Kawai M;Date T;Yoshimura M
• 通讯作者: Yoshimura M

Transient decrease in serum potassium level during ischemic attack of acute coronary syndrome: paradoxical contribution of plasma glucose level and glycohemoglobin.

• DOI: 10.1186/1475-2840-12-4
• 发表时间: 2013-01-04
• 期刊: Cardiovascular diabetology
• 影响因子: 9.3
• 作者: Sekiyama H;Nagoshi T;Komukai K;Matsushima M;Katoh D;Ogawa K;Minai K;Ogawa T;Yoshimura M
• 通讯作者: Yoshimura M

Cardiac tamponade as an independent condition affecting the relationship between the plasma B-type natriuretic peptide levels and cardiac function

心包填塞作为影响血浆B型利钠肽水平与心功能关系的独立病症

• DOI: 10.1007/s00380-012-0278-x
• 发表时间: 2013
• 期刊: Heart and Vessels
• 影响因子: 1.5
• 作者: Kosuke Minai;K. Komukai;S. Arase;T. Nagoshi;Seiichiro Matsuo;Kazuo Ogawa;Y. Kayama;K. Inada;Shin;Tomoyuki Takemoto;H. Sekiyama;T. Date;Takayuki Ogawa;I. Taniguchi;M. Yoshimura
• 通讯作者: M. Yoshimura

Aldosterone Preconditioning is Protective during Ischemia-Reperfusion through Modulation of p38 MAPK Signaling in Isolated Rat Hearts.

醛固酮预处理通过调节离体大鼠心脏中的 p38 MAPK 信号传导在缺血再灌注过程中发挥保护作用。

• 作者: 吉野 拓哉;吉村 道博;他
• 通讯作者: 他