Analysis of microRNA for the diagnosis and treatment of lung cancer

microRNA分析在肺癌诊断和治疗中的应用

• 批准号: 23591144
• 负责人: KONDO Masashi
• 金额: $ 3.33万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591144/
• 关键词: マイクロリボ核酸; 肺癌

MicroRNA-127 (miR-127) is located in 14q32 region and the deletion in this region has been reported in lung cancer as well as gastric cancer and hepatocellular carcinoma. The reduced expressions of miR-127 were observed in most of lung cancer cell lines, compared the immortalized normal human bronchial epithelial cell by real time PCR method with Taqman microRNA assay. The overexpression of miR-127 in two lung cancer cell lines, leaded to tumor growth inhibition. Moreover, mRNA microarray analysis of H1975 cancer cell lines after transfection of miR-127 mimic indicated the candidate target genes of miR-127. These results suggested that miR-127 have tumor suppressive effect in non-small-cell lung cancer.

microRNA-127（miR-127）位于14 q32区域，该区域的缺失在肺癌、胃癌和肝细胞癌中均有报道。 采用真实的时间PCR法和Taqman microRNA检测法比较永生化人支气管上皮细胞，发现miR-127在绝大多数肺癌细胞系中表达降低。 miR-127在两种肺癌细胞系中过表达，导致肿瘤生长抑制。 此外，在转染miR-127模拟物后的H1975癌细胞系的mRNA微阵列分析指示了miR-127的候选靶基因。 这些结果提示miR-127在非小细胞肺癌中具有抑瘤作用。

项目成果

The circadian clock gene BMAL1 is a novel therapeutic target for malignant pleural mesothelioma.

• DOI: 10.1002/ijc.27598
• 发表时间: 2012-12-15
• 期刊: INTERNATIONAL JOURNAL OF CANCER
• 影响因子: 6.4
• 作者: Elshazley, Momen;Sato, Mitsuo;Hase, Tetsunari;Yamashita, Ryo;Yoshida, Kenya;Toyokuni, Shinya;Ishiguro, Futoshi;Osada, Hirotaka;Sekido, Yoshitaka;Yokoi, Kohei;Usami, Noriyasu;Shames, David S.;Kondo, Masashi;Gazdar, Adi F.;Minna, John D.;Hasegawa, Yoshinori
• 通讯作者: Hasegawa, Yoshinori

Transient but not stable ZEB1 knockdown dramatically inhibits growth of malignant pleural mesothelioma cells.

瞬态但不稳定的Zeb1敲低会大大抑制恶性胸膜间皮瘤细胞的生长。

• DOI: 10.1245/s10434-011-2142-0
• 发表时间: 2012-07
• 期刊: Annals of surgical oncology
• 影响因子: 3.7
• 作者: Horio M;Sato M;Takeyama Y;Elshazley M;Yamashita R;Hase T;Yoshida K;Usami N;Yokoi K;Sekido Y;Kondo M;Toyokuni S;Gazdar AF;Minna JD;Hasegawa Y
• 通讯作者: Hasegawa Y

GENETIC POLYMORPHISMS OF THE ADENOSINE TRIPHOSPHATE-BINDING CASSETTE TRANSPORTERS(ABCG2, ABCB1) AND GEFITINIB TOXICITY

• DOI: 10.18999/nagjms.74.1-2.133
• 发表时间: 2012-02
• 期刊: Nagoya Journal of Medical Science
• 影响因子: 0.9
• 作者: Mariko Tamura;M. Kondo;Mihoko Horio;M. Ando;H. Saito;Masashi Yamamoto;Y. Horio;Y. Hasegawa

Functional analysis of miR-127 in non-small-cell lung cancer 非小細胞肺癌におけるmiR-127の機能解析

miR-127在非小细胞肺癌中的功能分析 miR-127在非小细胞肺癌中的功能分析

• 发表时间: 2014
• 作者: 丸山 英一;佐藤 光夫;近藤 征史
• 通讯作者: 近藤 征史

時計遺伝子Bmal1ノックダウンはアポトーシスと細胞老化を介して肺癌細胞の増殖を抑性する

时钟基因 Bmal1 敲低通过细胞凋亡和细胞衰老抑制肺癌细胞增殖

• 发表时间: 2013
• 作者: 山下良;佐藤光夫;Momen Elshazley;長谷哲成;丸山英一;梶川茂久;各務智彦;近藤征史;長谷川好規
• 通讯作者: 長谷川好規