Functional analysis of cancer stem cell/side population in non-small lung cancer cells

非小细胞肺癌细胞中癌症干细胞/旁群的功能分析

• 批准号: 20590918
• 负责人: KONDO Masashi
• 金额: $ 3.08万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590918/
• 关键词: EGFR; 癌幹細胞; 非小細胞肺癌; 肺癌; 癌幹細; EGFR遺伝子変異

Functional analysis of cancer stem cell is very important to establish the method of early detection and/or effective therapy for the non-small cell lung cancer since stem cells seem to be involved carcinogenesis and the resistance for the chemotherapy. In this study, we try to identify the cell populations that have cancer stem cell property and important molecule related with stem cells. We fund that the formation of sphere in serum free and anchorage-dependent culture condition was promising for the selection of stem cells population, and that epithelial cell adhesion molecule (EpCAM) play the important roll in survival of cancer cells.

癌症干细胞的功能分析对于建立非小细胞肺癌的早期检测和/或有效治疗方法非常重要，因为干细胞似乎与癌发生和化疗耐药有关。在这项研究中，我们试图鉴定具有癌症干细胞特性的细胞群以及与干细胞相关的重要分子。我们发现，在无血清和贴壁依赖性培养条件下球体的形成有望用于干细胞群的选择，并且上皮细胞粘附分子（EpCAM）在癌细胞的存活中发挥重要作用。

项目成果

Knockdown of ZEB1, a master epithelial-to-mesenchymal transition (EMT) gene, suppresses anchorage-independent cell growth of lung cancer cells.

• DOI: 10.1016/j.canlet.2010.04.008
• 发表时间: 2010-10-28
• 期刊: CANCER LETTERS
• 影响因子: 9.7
• 作者: Takeyama, Yoshihiro;Sato, Mitsuo;Horio, Mihoko;Hase, Tetsunari;Yoshida, Kenya;Yokoyama, Toshihiko;Nakashima, Harunori;Hashimoto, Naozumi;Sekido, Yoshitaka;Gazdar, Adi F.;Minna, John D.;Kondo, Masashi;Hasegawa, Yoshinori
• 通讯作者: Hasegawa, Yoshinori

Knockdown of ZEB1, a master EMT inducing gene, suppresses, anchorage-independent cell growth of lung cancer cells

ZEB1（一种主要的 EMT 诱导基因）的敲低可抑制肺癌细胞的贴壁依赖性细胞生长

• 发表时间: 2010
• 作者: Mitsuo Sato ;Yoshihiro Takeyama ;Mihoko Horio;Tetsunari Hase;Kenya Yoshida1,Harunori Nakashima;Naozumi Hashimoto;Yoshitaka Sekido;Masashi Kondo;Yoshinori Hasegawa
• 通讯作者: Yoshinori Hasegawa

Phase II study of carboplatin and gemcitabine as adjuvant chemotherapy in patients with completely resected non-small cell lung cancer: a report from the Central Japan Lung Study Group, CJLSG 0503 trial

• DOI: 10.1007/s10147-010-0118-x
• 发表时间: 2010-12-01
• 期刊: INTERNATIONAL JOURNAL OF CLINICAL ONCOLOGY
• 影响因子: 3.3
• 作者: Usami, Noriyasu;Yokoi, Kohei;Shimokata, Kaoru
• 通讯作者: Shimokata, Kaoru

Prospective evaluation of pharmacokinetically guided dosing of carboplatin in Japanese patients with cancer

• DOI: 10.1111/j.1349-7006.2010.01729.x
• 发表时间: 2010-12-01
• 期刊: CANCER SCIENCE
• 影响因子: 5.7
• 作者: Shimokata, Tomoya;Ando, Yuichi;Hasegawa, Yoshinori
• 通讯作者: Hasegawa, Yoshinori

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吉非替尼不良事件与ABC转运蛋白基因多态性的相关性

• 发表时间: 2008
• 作者: 田村真理子;近藤征史;ら
• 通讯作者: ら