An analysis of urinary microRNAs and proteomes in renal diseases.

我们检测了5名健康人和71例各种肾脏疾病患者的尿微RNA(UmiRNAs)谱。肾脏疾病患者尿中miRNAs浓度较健康对照组升高。芯片分析检测到83-137个不同的UmiRNAs和80-99%的UmiRNAs在健康对照组和肾脏疾病患者中都是如此。大多数肾脏疾病患者UmiRNAs的信号强度高于健康对照组，其中39个miRNAs的信号强度是健康对照组的100倍。在每种类型的肾脏疾病中观察到不同的UmiRNAs模式。肾组织和尿样的miRNA信号强度显著相关。这项研究表明，UmiRNAs呈现出与肾脏疾病类型相对应的不同模式。这些结果表明，UmiRNAs可能被用作肾脏疾病的新的生物标志物。

We examined the profiles of urinary microRNAs (UmiRNAs) in 5 healthy controls and 71 patients with various renal diseases. Urinary concentration of miRNAs increased in patients with renal disease compared with healthy controls. The microarray analysis detected 83-137 distinct UmiRNAs and 80-99% of UmiRNAs in both the healthy controls and the renal disease patients. The majority of UmiRNAs displayed higher signal intensity in renal disease patients than in healthy controls, including 39 miRNAs exhibiting signal intensities 100 times greater than that in healthy controls. A different pattern of UmiRNAs was observed in each type of renal disease. The miRNA signal intensity of renal tissue and urine samples was significantly correlated. This study demonstrated that UmiRNAs presented different patterns corresponding to the type of renal disease. These results suggest that UmiRNAs can potentially be used as novel biomarkers for renal diseases.