Molecular Mechanism for Phosphate Sensing and Bone-Kidney Functional Interaction

磷酸盐传感和骨肾功能相互作用的分子机制

• 批准号: 23591227
• 负责人: MICHIGAMI Toshimi
• 金额: $ 3.33万
• 依托单位: Research Institute, Osaka Medical Center for Maternal and Child Health
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591227/
• 关键词: リン代謝; 骨細胞; FGF23; DMP1; PHEX; ビタミンD; FAM20C

Recent studies suggest the roles of osteocyte in phosphate metabolism, although the underlying mechanisms remain unclear. In the current study, the roles of osteocytes in phosphate metabolism were investigated using the osteoblasts and osteocytes isolated from mouse long bones. The expression of Fgf23, Dmp1, Phex and Fam20c was higher in osteocytes than osteoblasts. In the osteocytes isolated from Phex-deficient Hyp mice, the expressions of Dmp1, Fam20c and Pit1 as well as that of Fgf23 were up-regulated. 24-hour treatment with 1,25-dihydroxyvitamin D markedly increased the expression of Fgf23. On the other hand, 24-hour treatment with high phosphate did not alter the expression of Fgf23, although it markedly increased the expression of Dmp1. These results suggest that chronic phosphate load is required for the increase of FGF23 production.

最近的研究表明骨细胞在磷代谢中的作用，但其机制尚不清楚。在本研究中，骨细胞在磷酸盐代谢中的作用进行了研究，使用成骨细胞和骨细胞从小鼠长骨分离。Fgf 23、Dmp 1、Phex和Fam 20 c在成骨细胞中的表达高于成骨细胞。在Phex缺陷Hyp小鼠的骨细胞中，Dmp 1、Fam 20 c和Pit 1以及Fgf 23的表达上调。24-用1，25-二羟维生素D处理1h显著增加Fgf 23的表达。另一方面，24小时的高磷酸盐处理并没有改变Fgf 23的表达，虽然它显着增加Dmp 1的表达。这些结果表明，慢性磷酸盐负荷是增加FGF 23产量所必需的。

项目成果

2013. Sympathetic activation induces skeletal Fgf23 expression in a circadian rhythm dependent manner

2013.交感神经激活以昼夜节律依赖性方式诱导骨骼Fgf23表达

• 发表时间: 2013
• 作者: Kawai M;Kinoshita S;Shimba S;Ozono K;Michigami T
• 通讯作者: Michigami T

1,25(OH)_2D and PTH up-regulate Rankl while down-regulate Phex and dmp1 in primary osteocytes isolated from mouse bones

1,25(OH)_2D 和 PTH 上调 Rankl，同时下调从小鼠骨骼中分离的原代骨细胞中的 Phex 和 dmp1

• 发表时间: 2011
• 作者: Miyagawa K;Ozono K;Tachikawa K;Kawai M;Mikuni-Takagaki Y;Kogo M;Michigami T
• 通讯作者: Michigami T

Circulating Levels of Soluble α-Klotho Are Markedly Elevated in Human Umbilical Cord Blood

• DOI: 10.1210/jc.2010-2357
• 发表时间: 2011-06-01
• 期刊: JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
• 影响因子: 5.8
• 作者: Ohata, Yasuhisa;Arahori, Hitomi;Ozono, Keiichi
• 通讯作者: Ozono, Keiichi

FGF23は可溶型klotho存在下で軟骨細胞の増殖・成熟を抑制する

FGF23 在可溶性 klotho 存在下抑制软骨细胞增殖和成熟

• 发表时间: 2012
• 作者: 天野慶子;十九浦るり子;小林加奈;大野弘毅;島田勝;猪上剛敏;街稔;清水淑子;種本雅之;内田俊也;山﨑美和,宮川和晃,大幡泰久,川井正信,大薗惠一,道上敏美;川井正信,木下さおり,大幡泰久,宮川和晃,山﨑美和,大薗惠一,道上敏美
• 通讯作者: 川井正信,木下さおり,大幡泰久,宮川和晃,山﨑美和,大薗惠一,道上敏美

脂溶性ビタミン

脂溶性维生素

• 作者: Yukiko Yasuoka;Katsumasa Kawahara;道上敏美
• 通讯作者: 道上敏美