Analysis of HLA-DP-specific immune responses to develop a novel immunotherapy

20 ~ 99%的白血病细胞HLA-DP表达阳性。从7例接受hla错配造血干细胞移植的患者的血液中分离出24株细胞毒性T细胞（CTL）。所有ctl均能识别hla I类或DR分子，但不能识别DP分子。在8例多次输血患者的血液中检测到抗hla抗体。所有抗体都能识别HLA I类分子，但不能识别HLA II类分子。体外实验表明，抗hla抗体可通过ADCC活性裂解造血干细胞。刺激T细胞。正在进行221个细胞转染每个HLA-DP cDNA构建。

The 20-99% of leukemia cells was positive for HLA-DP expression. Twenty-four cytotoxic T cell (CTL) lines were isolated from blood obtained from seven patients who had received HLA-mismatched hematopoietic stem cell transplantation. All CTLs recognized HLA-class I or DR molecules but not DP molecule. Anti-HLA antibodies were detected in blood obtained from eight patients who had received multi-transfusion. All antibodies recognized HLA class I but not class II molecules. In vitro assay using patient serum containing anti-HLA antibody demonstrated that the hematopoietic stem cells were lysed by anti-HLA antibody through ADCC activity. Stimulation of T cells by .221 cells transfected with each HLA-DP cDNA construct is ongoing.