Development of the new Helicobacter pylori eradication method for using a phage lytic enzyme

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To develop a novel eradication method of Helicobater pylori using bacteriolytic activity of a bacteriophage (phage) KHP30, the morphological, physical, and genetic aspects and the life-cycle of KHP30 have been studied. The host-range of KHP30 was about 65%, suggesting that it is suitable for application use as antibacterial agent. The complete DNA nucleotide sequence was determined and the open reading frames (ORFs) were annotated. The genome consisted of 26 kbp including 30 ORFs. By homology search of the genome, the ORF for the holin protein, one component of general lytic system, was found, but the ORF for the lysin, the counterpart of the system was not found. Therefore, H. pylori phage KHP30 was supposed to have a unique bacteriolytic system different from those of other phages.