Study on the epileptogenesis in child-onset intractable epilepsy

儿童难治性癫痫发病机制研究

• 批准号: 23591521
• 负责人: HAYASHI Masaharu
• 金额: $ 3.33万
• 依托单位: Tokyo Metropolitan Institute of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591521/
• 关键词: 医療・福祉; 臨床; 脳・神経; 病理学; 生体試料; 発達障害; 難治てんかん; 脳症

In order to clarify the epileptogenesis in child-onset intractable epilepsy, we performed the immunohistochemical analysis in autopsy brains, and the examination of amino acids and neurotransmitter metabolites in the cerebrospinal fluid from patients, simultaneously. We succeeded in disclosing the abnormal expression of voltage-dependent sodium channels and/or the facilitation of autophagy in the cerebral cortex in cases of West syndrome, and the disturbed metabolism of amino acid and monoamines in patients with infantile convulsion.In addition, we established the screening method of anti-neuronal antibodies in patients with subacute autoimmune encephalitis, which became to occur frequently in Japan, and explored the mechanisms of generation of refractory epilepsy in such encephalitis.We believe that these data will give us a clue for developing new treatments in patients with refractory epilepsy.

为了阐明儿童难治性癫痫的发病机制，我们对尸检脑组织进行了免疫组织化学分析，同时对患者脑脊液中的氨基酸和神经递质代谢产物进行了检测。我们成功地揭示了West综合征患者大脑皮层中电压依赖性钠通道的异常表达和/或自噬的易化，以及婴儿惊厥患者氨基酸和单胺代谢的紊乱。此外，我们建立了在日本频繁发生的亚急性自身免疫性脑炎患者中抗神经元抗体的筛选方法，并探讨了难治性癫痫的发生机制，为难治性癫痫的治疗提供了线索。

项目成果

Lesions of acetylcholine neurons in refractory epilepsy.

• DOI: 10.5402/2012/404263
• 发表时间: 2012
• 期刊: ISRN neurology
• 作者: Hayashi M;Nakajima K;Miyata R;Tanuma N;Kodama T
• 通讯作者: Kodama T

抗大脳基底核抗体

抗基底神经节抗体

• 发表时间: 2013
• 期刊: BRAIN and NERVE
• 作者: Okumura A;et al.;林雅晴
• 通讯作者: 林雅晴

特別講演小児の急性脳炎・脳症の病態解明

专题讲座：阐明小儿急性脑炎及脑病的病理

• 发表时间: 2013
• 作者: Okumura A;et al. (8名省略);林雅晴
• 通讯作者: 林雅晴

脳炎・脳症の病理所見

脑炎/脑病的病理结果

• 发表时间: 2011
• 作者: Yoshizawa-Ogasawara A;Katsumata N;Horikawa R;Satoh M;Urakami T;Tanaka T.;Yamashita Y;林雅晴
• 通讯作者: 林雅晴

Oxidative stress in developmental brain disorders. In : Ahmad S, eds. Neurodegenerative diseases. Austin : Landes Bioscience(ISBN : 978-1-4614-0652-5/)

发育性脑疾病中的氧化应激。

• 发表时间: 2012
• 作者: Hayashi M;Tanuma N;Miyata R.
• 通讯作者: Miyata R.