Analysis of presenilin gamma-secretase function on Abeta and NICD generation

淀粉样蛋白β 42是一种高度聚集性和致病性分子，可引发阿尔茨海默病。淀粉样蛋白β 42由γ-分泌酶产生。在这项研究中，我们进行了体外研究，其中纯化的γ分泌酶和底物，包括淀粉样β 42混合。我们进行了酶动力学研究，并阐明了淀粉样β蛋白是如何产生的。我们还分析了淀粉样β 42抑制化合物（γ分泌酶调节剂）的功能。我们还研究了γ分泌酶诱导NICD的过程。

Amyloid beta42 is a highly aggregatable and pathogenic molecule which triggers Alzheimer disease. Amyloid beta42 is generated by gamma-secretase. In this study, we performed in vitro study in which purified gamma secretase and substrates including amyloid beta42 are mixed. We performed enzyme kinetics study and elucidated how amyloid beta is generated. We also analyzed how amyloid beta42 inhibiting compounds (gamma secretase modulators) function. We also investigated NICD generating process by gamma secretase.