Development of novel therapy to control the acute rejection in organ transplantation model using the dedifferentiated fat cells(DFAT).

使用去分化脂肪细胞（DFAT）开发新疗法来控制器官移植模型中的急性排斥反应。

• 批准号: 23591879
• 负责人: SUGTOU kiminobu
• 金额: $ 3.41万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591879/
• 关键词: 移植・再生医療; 再生医学; 小児外科; 脱分化脂肪細胞; 免疫拒絶; DFAT; 前駆脂肪細胞; 皮膚移植; 免疫抑制

We examined T cell proliferation assay by co-culturing the T cells extracted from spleens of mice and mouse DFAT. In result, it was possible to suppress the proliferation of T cells in a concentration dependent of the DFAT. Then we examined for the immunosuppressive effect of DFAT in vivo. At first, establish a skin replacement transplant model and ectopic cardiac transplant model, we examined the survival prolongation of graft by DFAT administration. However, we didn't observed survival prolongation of graft by DFAT administration. Immunosuppressive effect in vitro is present, but it was found that not resulting to suppress rejection in vivo.

我们通过共同培养从小鼠和小鼠DFAT的脾脏提取的T细胞来检查T细胞增殖测定法。因此，有可能抑制依赖于DFAT的浓度的T细胞的增殖。然后，我们检查了DFAT在体内的免疫抑制作用。首先，建立皮肤替代移植模型和异位心脏移植模型，我们检查了DFAT给药的移植物的存活延长。但是，我们没有观察到DFAT给药的移植物生存延长。存在体外的免疫抑制作用，但发现没有导致体内抑制排斥反应。