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Basic and clinical research of circulating DNA fragments as a biomarker in gastroenterological cancer.

Basic and clinical research of circulating DNA fragments as a biomarker in gastroenterological cancer.
循环 DNA 片段作为胃肠道癌症生物标志物的基础和临床研究。
批准号:
23591907
负责人:
OZAWA Soji
金额:
$3.49万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2011
资助国家:
日本
项目状态:
已结题
起止时间:
2011 至 2013

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中文摘要
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英文摘要
Human squamous cancer cells (TE-4) were inoculated subcutaneously in the bilateral back of BALB/c nude mice. Unilateral tumors or bilateral tumors were resected 3 weeks after inoculation as surgical models. And 5-FU (25mg/kg or 50mg/kg) was injected intraperitoneously 4 times 3 weeks after inoculation as chemotherapy models. Blood was collected and animals were sacrificed 5 weeks after inoculation. Serum was processed and used for DNA measurement using a real time PCR method. DNA concentrations (Alu 247 and Alu115) correlated with tumor volumes. Serum DNA seems to be a good biomarker for cancer staging and evaluating chemotherapeutic effects.
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