Basic and clinical research of circulating DNA fragments as a biomarker in gastroenterological cancer.

循环 DNA 片段作为胃肠道癌症生物标志物的基础和临床研究。

• 批准号: 23591907
• 负责人: OZAWA Soji
• 金额: $ 3.49万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591907/
• 关键词: 癌; 核酸; 外科; 遺伝子; 食道癌; 手術; 化学療法; バイオマーカー

Human squamous cancer cells (TE-4) were inoculated subcutaneously in the bilateral back of BALB/c nude mice. Unilateral tumors or bilateral tumors were resected 3 weeks after inoculation as surgical models. And 5-FU (25mg/kg or 50mg/kg) was injected intraperitoneously 4 times 3 weeks after inoculation as chemotherapy models. Blood was collected and animals were sacrificed 5 weeks after inoculation. Serum was processed and used for DNA measurement using a real time PCR method. DNA concentrations (Alu 247 and Alu115) correlated with tumor volumes. Serum DNA seems to be a good biomarker for cancer staging and evaluating chemotherapeutic effects.

将人鳞癌细胞(TE-4)接种于BALB/c裸鼠双侧背部皮下。接种后3周切除单侧或双侧肿瘤作为手术模型。5-FU(25 mg/kg或50 mg/kg)接种3周后4次腹腔注射作为化疗模型。接种后5周采血，处死动物。用实时定量聚合酶链式反应方法处理血清并用于DNA检测。DNA浓度(Alu247和Alu115)与肿瘤体积相关。血清DNA可作为肿瘤分期和评价化疗效果的良好生物标志物。