Cryo-therapy was indicated to induce tumor growth inhibition and immune reation

冷冻疗法可诱导肿瘤生长抑制和免疫反应

• 批准号: 23591985
• 负责人: OSADA Shinji
• 金额: $ 3.24万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591985/
• 关键词: 肝癌; 抗腫瘍免疫; 凍結治療; サイトカイン; 低侵襲治療; IL-2; TNF-α; 免疫賦活; 進行肝臓癌; 進行肝臓がん

In vivo experiments on mouse, the tumor growth was inhibited by cry-ablation treatment from 717.52 to 551.0 on two weeks and from 3147.1 to 2543.1 on four weeks, which compared to control with tumor size (mm3). To demonstrate the relation to immune reaction, IL-2 and TNF-a were evaluated. The n, the expression of IL-2 in the tumor tissue was detected on 12hours after first and second treatment and TNF-a was appeared on 12 hours after first treatment. And the reaction was not shown after third treatment.

在小鼠体内实验中，冷冻消融治疗对肿瘤生长的抑制作用在两周内从717.52降至551.0，在四周内从3147.1降至2543.1，与对照组相比，肿瘤大小（mm 3）。 为了证明与免疫反应的关系，评价了IL-2和TNF-α。第一次和第二次治疗后12小时肿瘤组织中检测到IL-2的表达，第一次治疗后12小时肿瘤组织中出现TNF-α。 第三次处理后无反应。